amyX
168
pullulanase (debranching enzyme)
locus
BSU_29930
Molecular weight
80.92 kDa
pI
5.8
function
starch degradation
product
pullulanase
essential
no
synonyms
amyX
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1523 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,061,651 3,063,807
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The protein
Catalyzed reaction/ biological activity
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen (according to UniProt)
Protein family
[wiki|glycosyl hydrolase 13 family] (according to UniProt)
Structure
[PDB|2E8Z] (complex with alpha cyclodextrin), [PDB|2E9B] (complex with maltose)
[AF|C0SPA0]
Paralogous protein(s)
[protein|52C8DE3C78B680C8883D1CD559CBC2D9ABFCC400|glgB]
Expression and Regulation
Operons
genes
[gene|29A38D244A07ED0973D0846B652238524F4DBC67|ytlP]-[gene|DB1491F13F8B55A360413E815E47A2B51D24395C|ytlQ]-[gene|ED4C1EA16FF96DA20F12B972CD2458FA3B49DDA5|ytlR]-[gene|7D783DD180D950A4B030BF5EA78CCE930A466A07|amyX]
description
[pubmed|22383849]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|26577401], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
Biological materials
Mutant
BKE29930 ([gene|7D783DD180D950A4B030BF5EA78CCE930A466A07|amyX]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGGAAGCTCTCTCCTCC, downstream forward: _UP4_TGACGAAGTTTGTGCTATAG
BKK29930 ([gene|7D783DD180D950A4B030BF5EA78CCE930A466A07|amyX]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGGAAGCTCTCTCCTCC, downstream forward: _UP4_TGACGAAGTTTGTGCTATAG
References
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Time of last update: 2024-04-19 03:01:52
Author of last update: Jstuelk