acuC

168

protein deacetylase for the control of [protein|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA] activity

locus: BSU_29710

Molecular weight: 42.83 kDa

pI: 5.06

Protein length: 387 aa

Gene length: 1164 bp

function: control of [protein|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA] activity

product: [protein|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA] deacetylase

essential: no

synonyms: acuC

List of homologs in different organisms, belongs to COG0123 (Galperin et al., 2021)

Gene

Coordinates: 3,041,392  3,042,555

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The protein

Catalyzed reaction/ biological activity

• deacetylates (and thereby activates) [protein|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA] (together with [protein|A0A6CB19191A251BB5600C18E039978A9A34933C|srtN])  [Pubmed|19136592]

Protein family

• histone deacetylase family (single member, according to UniProt)

Structure

• [PDB|1C3R] (from Aquifex aeolicus, 35% identity) [pubmed|10490031]
• [AF|P39067]

Expression and Regulation

Operons

• genes: [gene|17C3F56CE859BCD7A282C144C46CC92D3506ABF6|acuA]-[gene|FC9981CFB9D7EB8ACDA3032EC2F0538988F277AA|acuB]-[gene|1255813797A83D835E0656A2AAAD361B5BB2094B|acuC]

  description: [Pubmed|7934817]

  regulation

  • repressed by glucose (3.4-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|7913927,12850135]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|7913927], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7913927], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • the mRNA is quite stable (half-life 5 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• GP1209 (''[gene|1255813797A83D835E0656A2AAAD361B5BB2094B|acuC]''::''kan''), available in [wiki|Jörg Stülke]'s lab
• GP1213 (''[gene|A0A6CB19191A251BB5600C18E039978A9A34933C|srtN]''::''cat'')(''[gene|1255813797A83D835E0656A2AAAD361B5BB2094B|acuC]''::''kan''), available in [wiki|Jörg Stülke]'s lab
• 1A887 ( ''acuC''::''spc''), [Pubmed|16855235], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A887&Search=1A887 BGSC]
• BKE29710 ([gene|1255813797A83D835E0656A2AAAD361B5BB2094B|acuC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TCTCATAGCAGATCCCTTTG,  downstream forward: _UP4_CAAAGAACAAAGTAAAAAAC
• BKK29710 ([gene|1255813797A83D835E0656A2AAAD361B5BB2094B|acuC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TCTCATAGCAGATCCCTTTG,  downstream forward: _UP4_CAAAGAACAAAGTAAAAAAC

References

• 7913927,16855235,7934817,19136592,18487328,26098117,12884008,10490031