acuB

168

unknown

locus: BSU_29700

Molecular weight: 24.21 kDa

pI: 6.59

Protein length: 214 aa

Gene length: 645 bp

function: unknown

product: unknown

essential: no

synonyms: acuB

List of homologs in different organisms, belongs to COG3448 (Galperin et al., 2021)

Gene

Coordinates: 3,040,751 → 3,041,395

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The protein

[wiki|Domains]

• 2 [wiki|CBS domain]s (aa 7-66, aa 78-135) (according to UniProt)

Structure

• [PDB|5AWE] (from Thermus thermophilus, 30% identity) [pubmed|26936147]
• [AF|P39066]

[wiki|Localization]

• Cytoplasm (Homogeneous) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|17C3F56CE859BCD7A282C144C46CC92D3506ABF6|acuA]-[gene|FC9981CFB9D7EB8ACDA3032EC2F0538988F277AA|acuB]-[gene|1255813797A83D835E0656A2AAAD361B5BB2094B|acuC]

  description: [Pubmed|7934817]

  regulation

  • repressed by glucose (3.4-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|7913927,12850135]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|7913927], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7913927], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • the mRNA is quite stable (half-life 5 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• BKE29700 (Δ[gene|FC9981CFB9D7EB8ACDA3032EC2F0538988F277AA|acuB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATTTTTTCCCCTTTGT,  downstream forward: _UP4_CCGTCAGAGCAAAGGGATCT
• BKK29700 (Δ[gene|FC9981CFB9D7EB8ACDA3032EC2F0538988F277AA|acuB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATTTTTTCCCCTTTGT,  downstream forward: _UP4_CCGTCAGAGCAAAGGGATCT

Expression vectors

• pGP2922 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab

References

• 7913927,16855235,7934817,16479537,19136592,26936147,12884008