http://subtiwiki.uni-goettingen.de/wiki/api.php?action=feedcontributions&user=Adr&feedformat=atom
SubtiWiki - User contributions [en]
2024-03-29T06:48:27Z
User contributions
MediaWiki 1.30.0
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=ComC&diff=122869
ComC
2010-07-14T11:33:50Z
<p>Adr: /* Expression and regulation */</p>
<hr />
<div>* '''Description:''' late competence gene required for processing and translocation of [[ComGC]], [[ComGD]], [[ComGE]] and [[ComGG]] [http://www.ncbi.nlm.nih.gov/pubmed/9723928 PubMed]<br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''comC''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || processing protease<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || genetic transformation<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 26 kDa, 9.843 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 744 bp, 248 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoIIB]]'', ''[[folC]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14767&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|-<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:comC_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU28070<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/comC.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10323]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' peptidase A24 family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cell membrane (according to Swiss-Prot)<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P15378 P15378]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28070]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''comC'' {{PubMed|1694528}} <br />
<br />
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|1694528}} <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
** ComK: transcription activation {{PubMed|8196543}}<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>9723928,16751195,7783624,2553669,1694528, 8196543 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=YwiE&diff=121268
YwiE
2010-03-07T14:22:09Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' minor cardiolipin synthetase, general stress protein <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''ywiE''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || minor cardiolipin synthetase <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || phospholipiid biosynthesis<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 58 kDa, 8.384 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1500 bp, 500 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywjA]]'', ''[[narI]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15752&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:ywiE_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU37240<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11305]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' 2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' Cardiolipin synthase subfamily (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):''' [[ClsA]], [[YwjE]]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cell membrane (according to Swiss-Prot)<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P45860 P45860]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37240]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' <br />
<br />
* '''[[Sigma factor]]:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed] <br />
<br />
* '''Regulation:''' induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]<br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>14973018 , 15805528, 16514141 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=YwpB&diff=121267
YwpB
2010-03-07T14:22:04Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' β-hydroxyacyl-(acyl carrier protein) dehydratase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''ywpB''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || β-hydroxyacyl-ACP dehydratase <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 9.36 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 396 bp, 132 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mscL]]'', ''[[rapD]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15654&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|-<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:ywpB_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU36370<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12496]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:''' FabZ subfamily (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):''' [[YcsD]]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cytoplasm (according to Swiss-Prot)<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3D6X 3D6X] (from ''Campylobacter jejuni'', 45% identity, 67% similarity) {{PubMed|19280690}}<br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P94584 P94584]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36370]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' <br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<pubmed>17919287 19280690 </pubmed><br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=YwjE&diff=121266
YwjE
2010-03-07T14:21:59Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' minor cardiolipin synthetase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''ywjE''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || minor cardiolipin synthetase <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || phospholipid biosynthesis<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 9.053 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1194 bp, 398 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fadF]]'', ''[[ywjD]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15747&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:ywjE_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU37190<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11310]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:''' Cardiolipin synthase subfamily (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):''' [[ClsA]], [[YwiE]]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cell membrane (according to Swiss-Prot)<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P45865 P45865]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37190]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' <br />
<br />
* '''[[Sigma factor]]:''' [[SigG]] {{PubMed|15699190}}<br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>18820022,14973018 , 16514141 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=YwaA&diff=121265
YwaA
2010-03-07T14:21:54Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' branched-chain amino acid aminotransferase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''ywaA''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-0r ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || branched-chain amino acid aminotransferase <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of branched-chain amino acids<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 4.952 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1089 bp, 363 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dltE]]'', ''[[licH]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15881&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|-<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:ywaA_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU38550<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywaA.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10546]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):''' [[YbgE]]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:''' S-cysteinylation after diamide stress (C104) [http://www.ncbi.nlm.nih.gov/sites/entrez/17611193 PubMed]<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3HT5 3HT5] (from '' Mycobacterium tuberculosis'', 42% identity, 58% similarity) {{PubMed|19923721}} <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39576 P39576]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU38550]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.6.1.42 2.6.1.42]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''ywaA'' {{PubMed|9353933}}<br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>12670965,17611193,, </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=YqhY&diff=121264
YqhY
2010-03-07T14:21:46Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' unknown <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''yqhY''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/17114254 PubMed] <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 4.414 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 405 bp, 135 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nusB]]'', ''[[accC]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14364&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|-<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:yqhY_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU24330<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/17114254 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/accBC-yqhY.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11709]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:''' asp23 family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):''' [[YloU]]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P54519 P54519]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24330]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[accB]]-[[accC]]-[[yqhY]]'' {{PubMed|7592499}}<br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>17114254, 7592499 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=YhdN&diff=121263
YhdN
2010-03-07T14:21:40Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' general stress protein, aldo/keto reductase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''yhdN''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || aldo/keto reductase, specific for NADP <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 4.77 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 993 bp, 331 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sigM]]'', ''[[plsC]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12792&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:yhdN_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU09530<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yhdNO.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13020]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:''' aldo/keto reductase 2 family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1PZ1 1PZ1] <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P80874 P80874]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU09530]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[yhdN]]-[[plsC]]'' {{PubMed|10220166}} <br />
<br />
* '''[[Sigma factor]]:''' [[SigB]] {{PubMed|10220166,15805528}} <br />
<br />
* '''Regulation:''' <br />
** induced by stress ([[SigB]]) {{PubMed|10220166,15805528}}<br />
** induced upon malonyl-CoA limitation ([[FapR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[FapR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>10220166,15019785,,12737802,15805528, </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=MutY&diff=121262
MutY
2010-03-07T14:21:34Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' similar to A/G-specific adenine glycosylase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''yfhQ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || error prevention oxidized guanine system<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 6.044 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1107 bp, 369 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfhP]]'', ''[[yfhS]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12691&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:yfhQ_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU08630<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yfhQ-fabL-sspE.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12891]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:'''<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1RRS 1RRS] (complex with DNA, Geobacillus stearothermophilus) <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/O31584 O31584]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU08630]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[yfhQ]]-[[fabL]]-[[sspE]]'' {{PubMed|10463184}}<br />
<br />
* '''[[Sigma factor]]:''' <br />
**[[SigA]] {{PubMed|10463184}} <br />
**[[SigG]] {{PubMed|1900507}}<br />
<br />
* '''Regulation:''' <br />
** [[YfhP]] may act as a negative transcription regulator of the ''[[yfhQ]]-[[fabL]]-[[sspE]]'' operon during exponential growth {{PubMed|10463184}}<br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<pubmed>10463184 19011023 </pubmed><br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=YbgE&diff=121261
YbgE
2010-03-07T14:20:06Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' branched-chain amino acid aminotransferase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''ybgE''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || branched-chain amino acid aminotransferase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of branched-chain amino acids<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val], [http://subtiwiki.uni-goettingen.de/pathways/CoA_synthesis.html Coenzyme A]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 4.952 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1068 bp, 356 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ybgB]]'', ''[[ybgF]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12033&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:ybgE_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU02390<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ybgE.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12749]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' l-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):''' [[YwaA]]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/O31461 O31461]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU02390]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.6.1.42 2.6.1.42]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''ybgE'' {{PubMed|15060025}}<br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]<br />
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/18083814 PubMed2]<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:''' pGP248 (in [[pAC5]]), available in [[Stülke]] lab<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>18083814,12618455,12670965,,15060025 ,12107147, </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=YcsD&diff=121260
YcsD
2010-03-07T14:19:59Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' β-hydroxyacyl-(acyl carrier protein) dehydratase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''ycsD''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || β-hydroxyacyl-ACP dehydratase <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 4.687 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 390 bp, 130 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yczH]]'', ''[[ycsE]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12211&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:ycsD_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU04030<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ycsD.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11225]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:''' FabZ subfamily (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):''' [[YwpB]]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P42961 P42961]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU04030]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''ycsD'' (according to [http://dbtbs.hgc.jp/COG/prom/ycsD.html DBTBS])<br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<pubmed>17919287</pubmed><br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=YbfM&diff=121259
YbfM
2010-03-07T14:19:54Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' similar to alkaline phosphatase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''ybfM''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 9.482 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 486 bp, 162 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pssA]]'', ''[[psd]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12022&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:ybfM_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU02280<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pssA-ybfM-psd.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12741]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:''' dedA family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cell membrane (according to Swiss-Prot)<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/O31453 O31453]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU02280]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[pssA]]-[[ybfM]]-[[psd]]'' {{PubMed|14762009}} <br />
<br />
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|14762009}}, [[SigX]] {{PubMed|14762009}}<br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>14762009,, </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=UgtP&diff=121258
UgtP
2010-03-07T14:19:50Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' UDP-glucose diacylglycerol glucosyltransferase, growth-rate dpendent inhibitor of cell division <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''ugtP''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ypfP ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || UDP-glucose diacylglycerol glucosyltransferase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of glycolipids and anchoring of lipoteichoic <br/>acid, inhibition of [[FtsZ]] assembly<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 8.398 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1146 bp, 382 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[metA]]'', ''[[cspD]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14110&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:ugtP_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU21920<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11611]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' UDP-glucose + 1,2-diacylglycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol (according to Swiss-Prot) <br />
<br />
* '''Protein family:'''<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:''' [[EzrA]]-[[UgtP]], [[UgtP]]-[[UgtP]]<br />
<br />
* '''Localization:''' cytoplasm (according to Swiss-Prot), membrane-bound protein, self-assembles into tightly wound spirals ''in vitro'' {{PubMed|17662947}}<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P54166 P54166]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU21920]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' <br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
==Reviews==<br />
<pubmed> 19680248 </pubmed><br />
<br />
==Original Publications==<br />
<pubmed>9244290,18820022,17662947,9720862,, </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=TnrA&diff=121257
TnrA
2010-03-07T14:19:40Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' transcriptional pleiotropic regulator invoved in global nitrogen regulation <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''tnrA''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''scgR ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || transcription activator/ repressor<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' ||regulation of nitrogen assimilation <br/>(positive regulation of nrgAB, nasBCDEF, gabP,<br/> ureABC, guaD; negative regulation of glnRA, gltAB)<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val], <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate], [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://subtiwiki.uni-goettingen.de/pathways/cellwall.html Cell wall], <br/>[http://subtiwiki.uni-goettingen.de/pathways/CoA_synthesis.html Coenzyme A], [http://subtiwiki.uni-goettingen.de/pathways/phosphorelay.html Phosphorelay]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 10.235 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 330 bp, 110 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mgtE]]'', ''[[ykzB]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13188&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:tnrA_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU13310<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/tnrA.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11805]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:'''<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Genes/ operons controlled by TnrA ===<br />
<br />
* '''Activation by TnrA:''' ''[[nrgA]]-[[nrgB]]''<br />
<br />
* '''Repression by TnrA:''' ''[[degS]]-[[degU]]'', ''[[gltA]]-[[gltB]]''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:''' feedback-inhibited [[GlnA]] prevents TnrA from DNA binding<br />
<br />
* '''Interactions:''' [[TnrA]]-[[GlnA]], [[TnrA]]-[[NrgB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17001076 PubMed], [[TnrA]]-[[GlnA]], this interaction results in loss of TnrA DNA-binding activity<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/Q45666 Q45666]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13310]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''tnrA'' (according to [http://dbtbs.hgc.jp/COG/prom/tnrA.html DBTBS])<br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
** expression is autocativated (TnrA) and repressed by [[GlnR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16547045 PubMed]<br />
** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10671441,16547045 PubMed]<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[TnrA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/10671441,16547045 PubMed]<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:''' GP252 (in frame deletion), available in the [[Stülke]] lab<br />
<br />
* '''Expression vector:'''<br />
** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP171 available in [[Stülke]] lab<br />
** pGP229 (N-terminal Strep-tag, for [[SPINE]], expression in ''B. subtilis'', in [[pGP380]]), available in [[Stülke]] lab<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:''' available in the [[Karl Forchhammer]] lab<br />
<br />
=Labs working on this gene/protein=<br />
<br />
[[Susan Fisher]], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed> 10231480 18086213 </pubmed><br />
==Original publications==<br />
<pubmed>12374841,15547269,9287005,12823818,12950915,10671441,16547045,11719184, 16547045 ,12139611, 8799114, 12823818,15150225, 11029411,17001076,15547269, 17085574 <br />
2573733, 8636055, 19233925, 16493705, 16885465, 6141156 18667567 </pubmed><br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=Spo0A&diff=121256
Spo0A
2010-03-07T14:19:23Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' [[phosphorelay]] regulator, initiation of sporulation <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''spo0A''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spo0C, spo0G, spoIIL, sof-1 ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || [[phosphorelay]] response regulator<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || initiation of sporulation<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/biofilm.html Biofilm], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)], [http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate], <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism],[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism], [http://subtiwiki.uni-goettingen.de/pathways/phosphorelay.html Phosphorelay], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress], <br/>[http://subtiwiki.uni-goettingen.de/pathways/tRNA_charging.html tRNA charging],[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis] '''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.989 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 801 bp, 267 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqiG]]'', ''[[spoIVB]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14353&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:spo0A_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU24220<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spo0A.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10765]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:'''<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Genes controlled by Spo0A ===<br />
<br />
* '''Activation by Spo0A:''' ''[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'', ''[[spoIIE]]'', ''[[spoIIGA]]-[[sigE]]-[[sigG]]''<br />
<br />
* '''Repression by Spo0A:''' ''[[abrB]], [[kinA]], [[kinC]], [[spo0A]]'', ''[[metS]]''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:''' receives phosphorylation from [[Spo0B]], dephosphorylation by [[Spo0E]], direct phosphorylation by [[KinC]] upon potassium leakage {{PubMed|19114652}}<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:''' the phosphorylation state affects DNA binding activity<br />
<br />
* '''Interactions:''' [[KinC]]-[[Spo0A]] {{PubMed|19114652}}, [[Spo0A]]-[[Spo0E]], [[Spo0A]]-[[SigA]], [[Spo0A]]-[[Spo0B]]<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1QMP 1QMP] (with phosphorylated aspartate, ''Geobacillus stearothermophilus''), [http://www.rcsb.org/pdb/explore.do?structureId=1FC3 1FC3] (trans-activation domain, ''Geobacillus stearothermophilus''), [http://www.rcsb.org/pdb/explore.do?structureId=1LQ1 1LQ1] (complex with DNA) <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P06534 P06534]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24220]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''spo0A'' {{PubMed|11254139}}<br />
<br />
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|11254139,1569009}}, [[SigH]] {{PubMed|11254139,1569009}}<br />
<br />
* '''Regulation:''' <br />
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1391039 PubMed]<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1391039 PubMed]<br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:''' <br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
* [[Tony Wilkinson]], York University, U.K. [http://www.york.ac.uk/depts/chem/staff/ajw.html homepage]<br />
* [[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]<br />
* [[Charles Moran]], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed>19995980 20030732 20157337 </pubmed><br />
<br />
==Original Publications==<br />
<pubmed>19528067, 19581368 , 8730857,2106683,3157992, 11254139,1569009, 12067336,10869437, 16385044,9287005, 8127878,9287022, 8231806, 1905258,16452424,2118505,8207022, 12176382, 9733708, 11069677,1391039,12730135, 2437099,8288522,16166384,9495766,2118512,1556084, 18296515,7768874, 3145384, 19114652 14651647,1537790,9658000, 8509330,14762002, 1846779, 2981817, 15687200, 9477965,8600030, 18840696, 1391039, 14679239, 14976210, 19207565 11679073 12270811 11112444 18978066 20154131 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=SspE&diff=121255
SspE
2010-03-07T14:18:29Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' small acid-soluble spore protein (major gamma-type SASP) <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''sspE''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || small acid-soluble spore protein (major gamma-type SASP)<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 9 kDa, 8.945 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 252 bp, 84 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fabL]]'', ''[[ygaB]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12694&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:sspE_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU08660<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yfhQ-fabL-sspE.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10789]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:''' gamma-type SASP family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P07784 P07784]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU08660]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon'''<br />
** ''[[yfhQ]]-[[fabL]]-[[sspE]]'' {{PubMed|10463184}}<br />
** ''[[fabL]]-[[sspE]]'' {{PubMed|10463184}}<br />
<br />
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|10463184}}, [[SigG]] {{PubMed|15699190}}<br />
<br />
* '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>3106326,1900507,2456528,2497051,11092849,,12107147,10463184 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=Spo0A&diff=121254
Spo0A
2010-03-07T14:18:22Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' [[phosphorelay]] regulator, initiation of sporulation <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''spo0A''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spo0C, spo0G, spoIIL, sof-1 ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || [[phosphorelay]] response regulator<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || initiation of sporulation<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/biofilm.html Biofilm], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)], [http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate], <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism],[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism], [http://subtiwiki.uni-goettingen.de/pathways/phosphorelay.html Phosphorelay], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress], </br>[http://subtiwiki.uni-goettingen.de/pathways/tRNA_charging.html tRNA charging],[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis] '''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.989 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 801 bp, 267 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqiG]]'', ''[[spoIVB]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14353&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:spo0A_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU24220<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spo0A.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10765]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:'''<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Genes controlled by Spo0A ===<br />
<br />
* '''Activation by Spo0A:''' ''[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'', ''[[spoIIE]]'', ''[[spoIIGA]]-[[sigE]]-[[sigG]]''<br />
<br />
* '''Repression by Spo0A:''' ''[[abrB]], [[kinA]], [[kinC]], [[spo0A]]'', ''[[metS]]''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:''' receives phosphorylation from [[Spo0B]], dephosphorylation by [[Spo0E]], direct phosphorylation by [[KinC]] upon potassium leakage {{PubMed|19114652}}<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:''' the phosphorylation state affects DNA binding activity<br />
<br />
* '''Interactions:''' [[KinC]]-[[Spo0A]] {{PubMed|19114652}}, [[Spo0A]]-[[Spo0E]], [[Spo0A]]-[[SigA]], [[Spo0A]]-[[Spo0B]]<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1QMP 1QMP] (with phosphorylated aspartate, ''Geobacillus stearothermophilus''), [http://www.rcsb.org/pdb/explore.do?structureId=1FC3 1FC3] (trans-activation domain, ''Geobacillus stearothermophilus''), [http://www.rcsb.org/pdb/explore.do?structureId=1LQ1 1LQ1] (complex with DNA) <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P06534 P06534]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24220]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''spo0A'' {{PubMed|11254139}}<br />
<br />
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|11254139,1569009}}, [[SigH]] {{PubMed|11254139,1569009}}<br />
<br />
* '''Regulation:''' <br />
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1391039 PubMed]<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1391039 PubMed]<br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:''' <br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
* [[Tony Wilkinson]], York University, U.K. [http://www.york.ac.uk/depts/chem/staff/ajw.html homepage]<br />
* [[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]<br />
* [[Charles Moran]], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed>19995980 20030732 20157337 </pubmed><br />
<br />
==Original Publications==<br />
<pubmed>19528067, 19581368 , 8730857,2106683,3157992, 11254139,1569009, 12067336,10869437, 16385044,9287005, 8127878,9287022, 8231806, 1905258,16452424,2118505,8207022, 12176382, 9733708, 11069677,1391039,12730135, 2437099,8288522,16166384,9495766,2118512,1556084, 18296515,7768874, 3145384, 19114652 14651647,1537790,9658000, 8509330,14762002, 1846779, 2981817, 15687200, 9477965,8600030, 18840696, 1391039, 14679239, 14976210, 19207565 11679073 12270811 11112444 18978066 20154131 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PssA&diff=121253
PssA
2010-03-07T14:16:59Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' phosphatidylserine synthase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''pssA''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''pss ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphatidylserine synthase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 7.881 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 531 bp, 177 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ybfK]]'', ''[[ybfM]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12021&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:pssA_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU02270<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pssA-ybfM-psd.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11012]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' CDP-diacylglycerol + L-serine = CMP + (3-sn-phosphatidyl)-L-serine (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' CDP-alcohol phosphatidyltransferase class-I family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cell membrane (according to Swiss-Prot)<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39823 P39823]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU02270]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.8.8 2.7.8.8]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[pssA]]-[[ybfM]]-[[psd]]'' {{PubMed|14762009}} <br />
<br />
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|14762009}}, [[SigX]] {{PubMed|14762009}}<br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed>9370336 , </pubmed><br />
==Original publications==<br />
<pubmed>14762009,18820022,9422599 ,8829529 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=Ptb&diff=121252
Ptb
2010-03-07T14:16:49Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' phosphate butyryltransferase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''ptb''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqiS, bkd ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphate butyryltransferase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of branched-chain keto acids<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 7.936 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 897 bp, 299 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bcd]]'', ''[[bkdR]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14340&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|-<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:ptb_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU24090<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptb-bcd-buk-lpdV-bkdAABB.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11722]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' Butanoyl-CoA + phosphate = CoA + butanoylphosphate (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=1YCO 1YCO] (from ''Enterococcus faecalis v583'', 36% identity, 56% similarity)<br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P54530 P54530]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24090]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.19 2.3.1.19]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
* '''Operon:''' ''[[ptb]]-[[bcd]]-[[buk]]-[[lpdV]]-[[bkdAA]]-[[bkdAB]]-[[bkdB]]'' {{PubMed|12823818}}<br />
<br />
* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]<br />
<br />
* '''Regulation:''' <br />
** induced in the presence of isoleucine or valine ([[BkdR]]) {{PubMed|10094682}}<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]<br />
** [[BkdR]]: transcription activation {{PubMed|10094682}}<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>12427936,15241682,12823818,10094682, </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=Psd&diff=121251
Psd
2010-03-07T14:16:43Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' phosphatidylserine decarboxylase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''psd''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphatidylserine decarboxylase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 7.27 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 789 bp, 263 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ybfM]]'', ''[[ybfN]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12023&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:psd_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU02290<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pssA-ybfM-psd.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11013]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' Phosphatidyl-L-serine = phosphatidylethanolamine + CO<sub>2</sub> (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' Type 1 subfamily (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39822 P39822]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU02290]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/4.1.1.65 4.1.1.65]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[pssA]]-[[ybfM]]-[[psd]]'' {{PubMed|14762009}} <br />
<br />
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|14762009}}, [[SigX]] {{PubMed|14762009}}<br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>14762009,18820022,9422599 , </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PlsY&diff=121250
PlsY
2010-03-07T14:16:37Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' acylphosphate:glycerol-phosphate acyltransferase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''plsY ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yneS''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || acylphosphate:glycerol-phosphate acyltransferase <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 10.408 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 579 bp, 193 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yneR]]'', ''[[yneT]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13690&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:yneS_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU18070<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11826]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' Acyl-phosphate + sn-glycerol 3-phosphate = acyl-sn-glycerol 3-phosphate + phosphate (according to UniProt)<br />
<br />
* '''Protein family:''' UPF0078 family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:''' [[PlsY]]-[[PlsX]] {{PubMed|19282621}}<br />
<br />
* '''Localization:''' cell membrane {{PubMed|19820159}}<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/Q45064 Q45064]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU18070]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' <br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in Matsumoto lab {{PubMed|19282621}}<br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed> 15952903 17919287</pubmed><br />
==Original Publications==<br />
<pubmed> 19282621, 17005971, 17557823, 19820159, </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PlsC&diff=121249
PlsC
2010-03-07T14:14:35Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' acyl-ACP:1-acylglycerolphosphate acyltransferase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''plsC''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yhdO''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || acyl-ACP:1-acylglycerolphosphate acyltransferase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 9.976 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 597 bp, 199 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhdN]]'', ''[[yhdP]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12793&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:yhdO_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU09540<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yhdNO.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13021]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:'''<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/O07584 O07584]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU09540]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.51 2.3.1.51]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[yhdN]]-[[plsC]]'' {{PubMed|10220166}} <br />
<br />
* '''[[Sigma factor]]:''' [[SigB]] {{PubMed|10220166,15805528}} <br />
<br />
* '''Regulation:''' <br />
** induced by stress ([[SigB]]) {{PubMed|10220166,15805528}}<br />
** induced upon malonyl-CoA limitation ([[FapR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[FapR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>12737802,17557823 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PlsX&diff=121248
PlsX
2010-03-07T14:14:30Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' acyl-acyl carrier protein (ACP):phosphate acyltransferase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''plsX''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylpD ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || acyl-acyl carrier protein (ACP):phosphate acyltransferase <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 5.492 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 999 bp, 333 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fapR]]'', ''[[fabD]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13462&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:plsX_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU15890<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fapR-plsX-fabDG.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11843]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:''' plsX family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:''' [[PlsX]]-[[PlsY]] {{PubMed|19282621}}<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1VI1 1VI1] <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P71018 P71018]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15890]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[fapR]]-[[plsX]]-[[fabD]]-[[fabG]]-[[acpA]]'' {{PubMed|12737802,19850612}}<br />
<br />
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|16932747}}<br />
<br />
* '''Regulation:''' <br />
** induced if the cells experience an accumulation of malonyl-CoA ([[FapR]]) {{PubMed|12737802}}<br />
** expressed at high cell density ([[ComA]]) {{PubMed|16091051}}<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[ComA]]: transcription activation ([[ComA]]) {{PubMed|16091051}}<br />
** [[FapR]]: transcription repression {{PubMed|12737802}}<br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in Matsumoto lab {{PubMed|19282621}}<br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>12737802, 19282621, 17645809, 17557823, 8759840 16932747 19850612 16091051</pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PgsA&diff=121247
PgsA
2010-03-07T14:14:22Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' phosphatidylglycerophosphate synthase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''pgsA''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ymfN ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphatidylglycerophosphate synthase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 5.163 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 579 bp, 193 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rodZ]]'', ''[[cinA]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13565&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|-<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:pgsA_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU16920<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11373]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' CDP-alcohol phosphatidyltransferase class-I family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cell membrane {{PubMed|19820159}} <br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P46322 P46322]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16920]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.8.5 2.7.8.5]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' <br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<pubmed>19745567 19820159 </pubmed><br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PgcA&diff=121246
PgcA
2010-03-07T14:14:15Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' alpha-phosphoglucomutase, required for UDP-glucose synthesis <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''pgcA''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yhxB''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || alpha-phosphoglucomutase <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || interconversion of glucose 6-phosphate and alpha-glucose 1-phosphate<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 62 kDa, 4.913 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1695 bp, 565 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glpD]]'', ''[[yhcY]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12759&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|-<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:yhxB_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU09310<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10189]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' phosphohexose mutase family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:''' phosphorylated on ser/ thr/ tyr [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed] <br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P18159 P18159]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU09310]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/5.4.2.2 5.4.2.2]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' <br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>,15175311,17726680 16493705, 15640167 17662947 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=MprF&diff=121245
MprF
2010-03-07T14:14:09Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' synthesis of lysylphosphatidylglycerol (L-PG) <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''mprF''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yfiW, yfiX''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || lysyl-phosphatidylglycerol synthase <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || lysyl-phosphatidylglycerol lipid biosynthesis<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfiV]]'', ''[[yfiY]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAX52582&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:yfiW_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU08425<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yfiWX.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12899 old_YfiW][http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12900 old_YfiX]<br />
<br />
=== Additional information===<br />
<br />
Gene was re-assembled due to sequencing error in original sequence (it encompasses the genes annotated as ''yfiX'' and ''yfiW'' in SubtiList) [http://www.ncbi.nlm.nih.gov/sites/entrez/12427923 PubMed]<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:'''<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/C0H3X7 C0H3X7]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU08425]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''mprF'' (according to [http://dbtbs.hgc.jp/COG/prom/yfiWX.html DBTBS])<br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>19164152,18820022, 19734140, 18305156 12427923 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=LtaS&diff=121244
LtaS
2010-03-07T14:14:04Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' lipoteichoic acid synthase<br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''ltaS''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yflE''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || major lipoteichoic acid synthase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of lipoteichoic acid <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 74 kDa, 5.202 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1947 bp, 649 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nagP]]'', ''[[yflD]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12600&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:yflE_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:'''<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yflE.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12940]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:''' LTA synthase family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):''' [[YfnI]], [[YqgS]], [[YvgJ]]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:''' phosphorylated on Thr-297 {{PubMed|19229300}}<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cell membrane (according to Swiss-Prot), extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=69910&ordinalpos=1&log$=structuread_title 2W8D] <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34952 O34952]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU07710]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''ltaS'' (according to [http://dbtbs.hgc.jp/COG/prom/yflE.html DBTBS])<br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>19229300,17483484 , 19168632 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=GpsA&diff=121243
GpsA
2010-03-07T14:12:29Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' glycerol-3-phosphate dehydrogenase (NAD) <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''gpsA''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || glycerol-3-phosphate dehydrogenase (NAD)<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 6.231 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1035 bp, 345 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yphE]]'', ''[[yphC]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14199&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:gpsA_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU22830<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11366]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' sn-glycerol 3-phosphate + NAD(P)<sup>+</sup> = glycerone phosphate + NAD(P)H (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' NAD-dependent glycerol-3-phosphate dehydrogenase family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cell membrane (according to Swiss-Prot)<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3K96 3K96] (from ''Coxiella burnetii RSA 493'', 37% identity, 59% similarity)<br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P46919 P46919]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22830]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.94 1.1.1.94]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' <br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<pubmed> 7592341 </pubmed><br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=FapR&diff=121242
FapR
2010-03-07T14:12:24Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' repressor of fatty acid synthetic genes <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''fapR ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylpC''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of fatty acid biosynthesis<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 5.393 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 564 bp, 188 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[recG]]'', ''[[plsX]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13461&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:ylpC_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU15880<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fapR-plsX-fabDG.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13400]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' FapR regulates the expression of at least 10 genes (the ''fap'' regulon). It autoregulates its own expression. Malonyl-CoA, a precursor of fatty acid biosynthesis, binds to FapR changing its conformation to a non-DNA binding state. Hence, conditions that cause malonyl-CoA accumulation, like fatty acid biosynthesis inhibition, derepress the ''fap'' regulon.<br />
<br />
* '''Protein family:''' fapR family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:''' malonyl-CoA and malonyl-[[AcpA|ACP]] act as the molecular inducer of the FapR regulon {{PubMed|20201588}}<br />
<br />
* '''Interactions:''' malonyl-[[AcpA|ACP]]-[[FapR]] {{PubMed|20201588}}<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2F3X 2F3X] (complex with effector), [http://www.rcsb.org/pdb/explore.do?structureId=2F41 2F41] <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34835 O34835]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15880]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[fapR]]-[[plsX]]-[[fabD]]-[[fabG]]-[[acpA]]'' {{PubMed|12737802,19850612}}<br />
<br />
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|16932747}}<br />
<br />
* '''Regulation:''' <br />
** repressed in the absence of malonyl-CoA or malonyl-[[AcpA|ACP]] ([[FapR]]) {{PubMed|20201588}}<br />
** expressed at high cell density ([[ComA]]) {{PubMed|16091051}}<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[ComA]]: transcription activation ([[ComA]]) {{PubMed|16091051}}<br />
** [[FapR]]: transcription repression {{PubMed|12737802}}<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>12737802, 18941141, 18820022, 18384517, 17919287, 16932747,12737802 19850612 16091051 20201588</pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=FabL&diff=121241
FabL
2010-03-07T14:12:15Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' enoyl-acyl carrier protein reductase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''fabL''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yfhR, ygaA ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || enoyl-acyl carrier protein reductase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.967 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 750 bp, 250 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfhS]]'', ''[[sspE]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12693&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:fabL_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU08650<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yfhQ-fabL-sspE.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12221]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:'''<br />
<br />
* '''Paralogous protein(s):''' [[FabI]], one of the two proteins has to be present for viability [http://www.ncbi.nlm.nih.gov/sites/entrez/17114254 PubMed]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P71079 P71079]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU08650]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' <br />
** ''[[yfhQ]]-[[fabL]]-[[sspE]]'' {{PubMed|10463184}}<br />
** ''[[fabL]]-[[sspE]]'' {{PubMed|10463184}}<br />
<br />
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|10463184}}, [[SigG]] {{PubMed|15699190}}<br />
<br />
* '''Regulation:''' <br />
**repressed by [[YfhP]] during exponential growth {{PubMed|10463184}} <br />
**repressed by [[SpoVT]] during forespore expression {{PubMed|8755877}}<br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed> 15952903 17919287</pubmed><br />
==Original Publications==<br />
<pubmed>17114254, 10463184 15699190 8755877 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=FabI&diff=121240
FabI
2010-03-07T14:12:04Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' enoyl-acyl carrier protein reductase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''fabI''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yjbW ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || enoyl-acyl carrier protein reductase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.605 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 774 bp, 258 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[thiD]]'', ''[[cotO]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13029&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:fabI_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU11720<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fabI.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13152]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' Acyl-[acyl-carrier-protein] + NAD<sup>+</sup> = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' FabI subfamily (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):''' [[FabL]], one of the two proteins has to be present for viability [http://www.ncbi.nlm.nih.gov/sites/entrez/17114254 PubMed]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:''' <br />
** inhibited by triclosan {{PubMed|11325930}}<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1DFI 1DFI] (the enzyme from ''E. coli'' in complex with NAD) {{PubMed|8953047}}<br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P54616 P54616]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11720]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.3.1.9 1.3.1.9]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[fabI]]'' {{PubMed|12737802}}<br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
** expressed when the cells experience a lack of malonyl-CoA ([[FapR]]) {{PubMed|12737802}}<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[FapR]]: transcription repression {{PubMed|12737802}}<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed> 15952903 17919287</pubmed><br />
==Original Publications==<br />
<pubmed>12737802,17114254, 8953047 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=FabHB&diff=121239
FabHB
2010-03-07T14:11:55Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' beta-ketoacyl-acyl carrier protein synthase III <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''fabHB''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yhfB '', ''fabH2 ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || beta-ketoacyl-acyl carrier protein synthase III<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 5.684 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 975 bp, 325 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhgE]]'', ''[[yhfC]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12857&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:fabHB_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU10170<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fabHB.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13048]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacyl-[acyl-carrier-protein] + CoA + CO<sub>2</sub> (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' fabH family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):''' [[FabHA]], one of the two proteins has to be present for viability [http://www.ncbi.nlm.nih.gov/sites/entrez/17114254 PubMed]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cytoplasm (according to Swiss-Prot)<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EBL 1EBL] (FabH from ''E. coli'') {{PubMed|10673437}}<br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/O07600 O07600]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU10170]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.180 2.3.1.180], [http://www.expasy.org/enzyme/2.3.1.41 2.3.1.41]<br />
<br />
=== Additional information===<br />
* affinity for butyryl-CoA, but prefers acetyl-CoA in fatty acid biosynthesis {{PubMed|19820084}}<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''fabHB'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]<br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
** repressed unless the cells experience a lack of malonyl-CoA ([[FapR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[FapR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed> 15952903 17919287</pubmed><br />
==Original Publications==<br />
<pubmed>12737802,17114254,10629181, 10673437,19820084 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=FabHA&diff=121238
FabHA
2010-03-07T14:11:43Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' beta-ketoacyl-acyl carrier protein synthase III <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''fabHA''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yjaX '', ''fabH1 ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || beta-ketoacyl-acyl carrier protein synthase III<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 5.045 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 936 bp, 312 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yjzB]]'', ''[[fabF]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12974&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|-<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:fabHA_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU11330<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fabHAF.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13127]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacyl-[acyl-carrier-protein] + CoA + CO<sub>2</sub> (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' fabH family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):''' [[FabHB]], one of the two proteins has to be present for viability [http://www.ncbi.nlm.nih.gov/sites/entrez/17114254 PubMed]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cytoplasm (according to Swiss-Prot)<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EBL 1EBL] (FabH from ''E. coli'') {{PubMed|10673437}} <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34746 O34746]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11330]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.180 2.3.1.180], [http://www.expasy.org/enzyme/2.3.1.41 2.3.1.41]<br />
<br />
=== Additional information===<br />
* affinity for butyryl-CoA, but prefers acetyl-CoA in fatty acid biosynthesis {{PubMed|19820084}}<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[fabHA]]-[[fabF]]'' {{PubMed|12737802}}<br />
<br />
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|12737802}}<br />
<br />
* '''Regulation:''' <br />
** expressed when the cells experience a lack of malonyl-CoA ([[FapR]]) {{PubMed|12737802}}<br />
** inhibited by cerulenin {{PubMed|11325930}}<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[FapR]]: transcription repression {{PubMed|12737802}}<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed> 15952903 17919287</pubmed><br />
==Original Publications==<br />
<pubmed>12737802,17114254,10629181, 10673437,19820084 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=FabG&diff=121237
FabG
2010-03-07T14:10:34Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' beta-ketoacyl-acyl carrier protein reductase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''fabG''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylpF ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || beta-ketoacyl-acyl carrier protein reductase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 26 kDa, 8.091 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 738 bp, 246 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fabD]]'', ''[[acpA]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13464&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:fabG_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU15910<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fapR-plsX-fabDG.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11535]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' (3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP<sup>+</sup> = 3-oxoacyl-[acyl-carrier-protein] + NADPH (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1I01 1I01] (the enzyme from ''E. coli'') {{PubMed|11669613}} <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P51831 P51831]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15910]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.100 1.1.1.100]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[fapR]]-[[plsX]]-[[fabD]]-[[fabG]]-[[acpA]]'' {{PubMed|12737802,19850612}}<br />
<br />
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|16932747}}<br />
<br />
* '''Regulation:''' <br />
** induced if the cells experience an accumulation of malonyl-CoA ([[FapR]]) {{PubMed|12737802}}<br />
** expressed at high cell density ([[ComA]]) {{PubMed|16091051}}<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[ComA]]: transcription activation ([[ComA]]) {{PubMed|16091051}}<br />
** [[FapR]]: transcription repression {{PubMed|12737802}}<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed> 15952903 17919287</pubmed><br />
==Original Publications==<br />
<pubmed>12737802, 16932747 19850612 11669613 16091051</pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=FabF&diff=121236
FabF
2010-03-07T14:10:14Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' beta-ketoacyl-acyl carrier protein synthase II <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''fabF''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yjaY ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || beta-ketoacyl-acyl carrier protein synthase II<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 4.768 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1239 bp, 413 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fabHA]]'', ''[[yjaZ]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12975&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:fabF_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU11340<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fabHAF.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13128]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' (Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein] + CO<sub>2</sub> + [acyl-carrier-protein] (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' beta-ketoacyl-ACP synthases family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OX0 1OX0] (the protein from ''Streptococcus pneumoniae'') {{PubMed|12837788}} <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34340 O34340]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11340]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.179 2.3.1.179]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[fabHA]]-[[fabF]]'' {{PubMed|12737802}}<br />
<br />
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|12737802}}<br />
<br />
* '''Regulation:''' <br />
** expressed when the cells experience a lack of malonyl-CoA ([[FapR]]) {{PubMed|12737802}}<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[FapR]]: transcription repression {{PubMed|12737802}}<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed> 15952903 17919287</pubmed><br />
==Original Publications==<br />
<pubmed>18384517, 12837788,12737802, </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=CodY&diff=121235
CodY
2010-03-07T14:10:01Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' regulation of a large regulon in response to branched-chain amino acid limitation <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''codY''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional pleiotropic repressor<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of a large regulon in response to<br />
branched-chain amino acid limitation<br />
<br />
to the presence of branched-chain amino acids<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 4.75 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 777 bp, 259 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[clpY]]'', ''[[flgB]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13490&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:codY_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU16170<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
No swarming motility on B medium. [http://www.ncbi.nlm.nih.gov/sites/entrez/19202088 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/codV-clpQY-codY.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10968]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:''' codY family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Genes/ operons controlled by CodY ===<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' contains a GAF domain (ligand binding domain)<br />
<br />
* '''Modification:''' phosphorylation on Ser-215 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:''' GTP and branched chained amino acids (BCAA) increase the affinity of CodY for its DNA target sequences {{PubMed|11331605,15228537}}<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cytoplasm (according to Swiss-Prot)<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2B0L 2B0L] (C-terminal DNA-binding domain), [http://www.rcsb.org/pdb/explore.do?structureId=2GX5 2GX5] (N-terminal Gaf domain) <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39779 P39779]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16170]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[codV]]-[[clpQ]]-[[clpY]]-[[codY]]'' {{PubMed|7783641}} <br />
<br />
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7783641}}<br />
<br />
* '''Regulation:''' <br />
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11331605 PubMed]<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/11331605 PubMed]<br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:''' GP566, available in [[Stülke]] lab<br />
<br />
* '''Expression vector:'''<br />
** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP245, available in [[Stülke]] lab<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
[[Linc Sonenshein|Linc Sonenshein]], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]<br />
<br />
[[Tony Wilkinson]], York University, U.K. [http://www.york.ac.uk/depts/chem/staff/ajw.html homepage]<br />
<br />
[[Oscar Kuipers]], University of Groningen, The Netherlands<br />
[http://molgen.biol.rug.nl/molgen/index.php Homepage]<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>19542274,17493123,18641142,19202088,16995897,17982469,18083814,17993518,9287005,12618455,11331605,17218307,19500589,12591885,19202088,12618455,15802253,11331605,15228537,8793880,15937175,15916605,15916606,11331605,15228537 19749041 7783641</pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=FabD&diff=121234
FabD
2010-03-07T14:09:53Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' malonyl CoA-acyl carrier protein transacylase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''fabD''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylpE ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || malonyl CoA-acyl carrier protein transacylase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 4.515 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 951 bp, 317 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[plsX]]'', ''[[fabG]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13463&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:fabD_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU15900<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fapR-plsX-fabDG.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11836]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein] (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' fabD family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1MLA 1MLA] (the enzyme from ''E. coli'') {{PubMed|7768883}}<br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P71019 P71019]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15900]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.39 2.3.1.39]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[fapR]]-[[plsX]]-[[fabD]]-[[fabG]]-[[acpA]]'' {{PubMed|12737802,19850612}}<br />
<br />
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|16932747}}<br />
<br />
* '''Regulation:''' <br />
** induced if the cells experience an accumulation of malonyl-CoA ([[FapR]]) {{PubMed|12737802}}<br />
** expressed at high cell density ([[ComA]]) {{PubMed|16091051}}<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[ComA]]: transcription activation ([[ComA]]) {{PubMed|16091051}}<br />
** [[FapR]]: transcription repression {{PubMed|12737802}}<br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed> 15952903 </pubmed><br />
==Original Publications==<br />
<pubmed>12737802, 16932747 19850612 7768883 16091051</pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=ClsA&diff=121233
ClsA
2010-03-07T14:09:25Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' cardiolipin synthase, major enzyme <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''clsA''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywnE ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || cardiolipin synthase, major enzyme <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 55 kDa, 9.554 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1446 bp, 482 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywnF]]'', ''[[mta]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15676&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:ywnE_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU36590<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12483]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' 2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' Cardiolipin synthase subfamily (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):''' [[YwiE]], [[YwjE]]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cell membrane (according to Swiss-Prot)<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P71040 P71040]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36590]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' <br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>18820022,14973018 , </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=CdsA&diff=121232
CdsA
2010-03-07T14:09:19Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' phosphatidate cytidylyltransferase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''cdsA''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphatidate cytidylyltransferase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30 kDa, 7.866 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 807 bp, 269 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[uppS]]'', ''[[ispC]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13527&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:cdsA_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU16540<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12575]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' CTP + phosphatidate = diphosphate + CDP-diacylglycerol (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' CDS family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cell membrane {{PubMed|19820159}}<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/O31752 O31752]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16540]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.7.41 2.7.7.41]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' <br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>12884008,,19820159 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=BkdR&diff=121231
BkdR
2010-03-07T14:08:16Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' [[transcription factors activating transcription at SigL-dependent promoters |transcriptional activator]] of the ''[[ptb]]-[[bcd]]-[[buk]]-[[lpdV]]-[[bkdAA]]-[[bkdAB]]-[[bkdB]]'' operon <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''bkdR''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqiR ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || [[transcription factors activating transcription at SigL-dependent promoters |transcriptional activator]] (for [[SigL]]-dependent promoter))<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of branched-chain amino acid utilization, <br />
required for survival at low temperatures)<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 77 kDa, 6.237 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2076 bp, 692 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ptb]]'', ''[[yqzF]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14341&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:bkdR_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU24100<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
The mutant is cold-sensitive [http://www.ncbi.nlm.nih.gov/sites/entrez/16585774 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bkdR.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11721]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:'''<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:''' [[BkdR]]-[[SigL]] (activation)<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P54529 P54529]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24100]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''bkdR'' {{PubMed|12823818}} <br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]<br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>16585774,10094682,,12823818 , </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=Buk&diff=121230
Buk
2010-03-07T14:07:10Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' butyrate kinase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''buk''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqiU, bkd ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || butyrate kinase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of branched-chain keto acids<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 5.466 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1089 bp, 363 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[lpdV]]'', ''[[bcd]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14338&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:buk_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU24070<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptb-bcd-buk-lpdV-bkdAABB.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11724]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' ATP + butanoate = ADP + butanoyl phosphate (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' acetokinase family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cytoplasm (according to Swiss-Prot)<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=1X9J 1X9J] (from ''Thermotoga maritima'', 52% identity, 69% similarity)<br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P54532 P54532]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24070]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.2.7 2.7.2.7]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
* '''Operon:''' ''[[ptb]]-[[bcd]]-[[buk]]-[[lpdV]]-[[bkdAA]]-[[bkdAB]]-[[bkdB]]'' {{PubMed|12823818}}<br />
<br />
* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]<br />
<br />
* '''Regulation:''' <br />
** induced in the presence of isoleucine or valine ([[BkdR]]) {{PubMed|10094682}}<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]<br />
** [[BkdR]]: transcription activation {{PubMed|10094682}}<br />
<br />
* '''Additional information:'''<br />
<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>12427936,15241682,10094682,12823818, </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=BrnQ&diff=121229
BrnQ
2010-03-07T14:06:42Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' branched-chain amino acid [[transport proteins|transporter]]<br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''brnQ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yrdJ ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || branched-chain amino acid [[transport proteins|transporter]]<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || uptake of branched-chain amino acids <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46 kDa, 9.777 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1320 bp, 440 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yrdK]]'', ''[[azlD]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14610&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:brnQ_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU26690<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11918]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:''' branched chain amino acid transporter family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):''' [[BraB]]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P94499 P94499]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU26690]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[azlB]]-[[azlC]]-[[azlD]]-[[brnQ]]-[[yrdK]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9287000 PubMed]<br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' repressed by [[AzlB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9287000 PubMed]<br />
<br />
* '''Regulatory mechanism:''' [[AzlB]]: transcriptional repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9287000 PubMed]<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<pubmed> 9287000 </pubmed> <br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=BraB&diff=121228
BraB
2010-03-07T14:06:36Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' branched-chain amino acid [[transport proteins|transporter]] <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''braB''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || branched-chain amino acid [[transport proteins|transporter]]<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || uptake of branched-chain amino acids <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46 kDa, 9.65 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1335 bp, 445 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nifZ]]'', ''[[ezrA]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14938&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:braB_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU29600<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/braB.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12573]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:''' branched chain amino acid transporter family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):''' [[BrnQ]]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cell membrane (according to Swiss-Prot)<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34545 O34545]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29600]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[braB]]'' {{PubMed|9387221}} <br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<pubmed> 9387221 </pubmed><br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=Bcd&diff=121227
Bcd
2010-03-07T14:05:57Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''bcd''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''bkd, yqiT ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || valine dehydrogenase, isoleucine dehydrogenase, <br/>L-leucine dehydrogenase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of branched-chain keto acids<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 4.942 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1092 bp, 364 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[buk]]'', ''[[ptb]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14339&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:bcd_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU24080<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptb-bcd-buk-lpdV-bkdAABB.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11723]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' L-leucine + H<sub>2</sub>O + NAD<sup>+</sup> = 4-methyl-2-oxopentanoate + NH<sub>3</sub> + NADH (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=1LEH 1LEH] (from '' Lysinibacillus sphaericus'', 71% identity, 82% similarity) {{PubMed|8591046}} <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P54531 P54531]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24080]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.4.1.9 1.4.1.9]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
* '''Operon:''' ''[[ptb]]-[[bcd]]-[[buk]]-[[lpdV]]-[[bkdAA]]-[[bkdAB]]-[[bkdB]]'' {{PubMed|12823818}}<br />
<br />
* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]<br />
<br />
* '''Regulation:''' <br />
** induced in the presence of isoleucine or valine ([[BkdR]]) {{PubMed|10094682}}<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]<br />
** [[BkdR]]: transcription activation {{PubMed|10094682}}<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>12427936,15241682,10094682,12823818, </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=AzlD&diff=121226
AzlD
2010-03-07T14:05:51Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' branched-chain amino acid transport <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''azlD''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yrdI ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || branched-chain amino acid transport<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 9.962 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 330 bp, 110 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[brnQ]]'', ''[[azlC]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14611&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:azlD_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU26700<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11916]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:''' azlD/HI1737/HP1330 family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:'''<br />
<br />
* '''Localization:''' cell membrane (according to Swiss-Prot)<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/O07923 O07923]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU26700]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[azlB]]-[[azlC]]-[[azlD]]-[[brnQ]]-[[yrdK]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9287000 PubMed]<br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' repressed by [[AzlB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9287000 PubMed]<br />
<br />
* '''Regulatory mechanism:''' [[AzlB]]: transcriptional repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9287000 PubMed]<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>9287000,, </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=AcpS&diff=121225
AcpS
2010-03-07T14:05:44Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' acyl-carrier protein synthase, 4'-phosphopantetheine transferases <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''acpS''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ydcB ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || acyl-carrier protein synthase<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 13 kDa, 9.915 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 363 bp, 121 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ydcA]]'', ''[[ydcC]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12269&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:acpS_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU04620<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12089]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein] (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' AcpS family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):''' magnesium {{PubMed|11489886}}<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:''' [[AcpA]]-[[AcpS]] {{PubMed|10997907}}<br />
<br />
* '''Localization:''' cytoplasm (according to Swiss-Prot)<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1F7T 1F7T] {{PubMed|10997907}}, [http://www.rcsb.org/pdb/explore.do?structureId=1F80 1F80] (complex with [[AcpA]]) {{PubMed|10997907}}<br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P96618 P96618]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU04620]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.8.7 2.7.8.7]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' <br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed> 15952903 </pubmed><br />
==Original Publications==<br />
<pubmed>11489886,15955059,10997907,11867633,, </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=AcpA&diff=121224
AcpA
2010-03-07T14:05:33Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' acyl carrier protein <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''acpA''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''acpP ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || acyl carrier protein<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 8 kDa, 3.594 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 231 bp, 77 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fabG]]'', ''[[rnc]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13465&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:acpA_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU15920<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11536]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:'''<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:''' [[AcpA]]-[[AcpS]] {{PubMed|10997907}}, [[AcpA]]-[[BioI]] {{PubMed|18838690}}, malonyl-[[AcpA|ACP]]-[[FapR]] {{PubMed|20201588}}<br />
<br />
* '''Localization:''' cytoplasm (according to Swiss-Prot), during sporulation in the mother cell {{PubMed|19850612}}<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1F80 1F80] (complex with [[AcpS]]) {{PubMed|10997907}}, [http://www.rcsb.org/pdb/explore.do?structureId=3EJE 3EJE] (complex with [[BioI]]) {{PubMed|18838690}}, [http://www.rcsb.org/pdb/explore.do?structureId=1HY8 1HY8] <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P80643 P80643]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15920]<br />
<br />
* '''E.C. number:'''<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' <br />
** ''[[fapR]]-[[plsX]]-[[fabD]]-[[fabG]]-[[acpA]]'' {{PubMed|19850612}}<br />
** ''[[acpA]]'' {{PubMed|19850612}}<br />
<br />
* '''[[Sigma factor]]:''' <br />
** ''[[fapR]]'': [[SigA]] {{PubMed|16932747}}<br />
<br />
* '''Regulation:''' <br />
** ''[[fapR]]'': repressed in the absence of malonyl-CoA or malonyl-[[AcpA|ACP]] ([[FapR]]) {{PubMed|12737802}}<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[FapR]]: transcription repression {{PubMed|12737802}}<br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
* [[Diego de Mendoza]], Universidad Nacional de Rosario, Argentine [http://www.ibr.gov.ar/eng/investigacion/demendoza.htm homepage]<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed> 15952903 </pubmed><br />
==Original Publications==<br />
<pubmed>10997907,19850612, 12737802 18838690 20201588 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=AccA&diff=121223
AccA
2010-03-07T14:03:41Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' acetyl-CoA carboxylase (alpha subunit) <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''accA''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || acetyl-CoA carboxylase (alpha subunit))<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || production of malonyl-CoA, the substrate for fatty acid biosynthesis <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 6.087 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 975 bp, 325 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pfkA]]'', ''[[accD]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14880&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:accA_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU29200<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/accDA.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12557]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' ATP + acetyl-CoA + HCO<sub>3</sub><sup>-</sup> = ADP + phosphate + malonyl-CoA (according to Swiss-Prot) <br />
<br />
* '''Protein family:''' accA family (according to Swiss-Prot)<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:''' ([[AccA]]-[[AccD]])-[[AccB]]-[[AccC]] (acetyl-CoA carboxylase complex)<br />
<br />
* '''Localization:''' cytoplasm (according to Swiss-Prot), Membrane-proximal (Spotty) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OD2 1OD2] (carboxytransferase from yeast, equivalent to [[AccA]]-[[AccD]]) {{PubMed|12663926}}<br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34847 O34847]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29200]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/6.4.1.2 6.4.1.2]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[accD]]-[[accA]]'' (according to [http://dbtbs.hgc.jp/COG/prom/accDA.html DBTBS])<br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed> 15952903, 17919287 </pubmed><br />
<br />
==Original Publications==<br />
<pubmed>15066985,,14651647,16479537 12663926 </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=AccB&diff=121222
AccB
2010-03-07T14:03:29Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' acetyl-CoA carboxylase (biotin carboxyl carrier subunit) <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''accB''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''fabE, yqhW ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || acetyl-CoA carboxylase (biotin carboxyl carrier subunit)<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || production of malonyl-CoA, the substrate for fatty acid biosynthesis <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.394 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 477 bp, 159 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[accC]]'', ''[[spoIIIAH]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14366&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:accB_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU24350<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/accBC-yqhY.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11383]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:'''<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:''' ([[AccA]]-[[AccD]])-[[AccB]]-[[AccC]] (acetyl-CoA carboxylase complex)<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1BDO 1BDO] (the protein from ''E. coli'') {{PubMed|8747466}}<br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P49786 P49786]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24350]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/6.4.1.2 6.4.1.2]<br />
<br />
=== Additional information===<br />
<br />
AccB binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by [[SPINE]].<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[accB]]-[[accC]]-[[yqhY]]'' {{PubMed|7592499}}<br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed> 15952903 </pubmed><br />
==Original Publications==<br />
<pubmed>7592499, 8747466, </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=AccC&diff=121221
AccC
2010-03-07T14:03:17Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' acetyl-CoA carboxylase (biotin carboxylase subunit) <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''accC''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqhX ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || acetyl-CoA carboxylase (biotin carboxylase subunit)<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || production of malonyl-CoA, the substrate for fatty acid biosynthesis <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 4.72 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1350 bp, 450 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqhY]]'', ''[[accB]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14365&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|-<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:accC_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU24340<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/accBC-yqhY.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11384]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' ATP + biotin-carboxyl-carrier protein + CO<sub>2</sub> = ADP + phosphate + carboxybiotin-carboxyl-carrier protein (according to Swiss-Prot) <br />
<br />
* '''Protein family:'''<br />
<br />
* '''Paralogous protein(s):''' [[YotE]]<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:''' ([[AccA]]-[[AccD]])-[[AccB]]-[[AccC]] (acetyl-CoA carboxylase complex)<br />
<br />
* '''Localization:'''<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1BNC 1BNC] (the protein from ''E. coli'') {{PubMed|7915138}} <br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/P49787 P49787]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24340]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/6.3.4.14 6.3.4.14], [http://www.expasy.org/enzyme/6.4.1.2 6.4.1.2]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''[[accB]]-[[accC]]-[[yqhY]]'' {{PubMed|7592499}}<br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
<br />
* '''Regulatory mechanism:''' <br />
<br />
* '''Additional information:''' <br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed> 15952903 </pubmed><br />
==Original Publications==<br />
<pubmed> 7592499, 7915138, </pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=AccD&diff=121220
AccD
2010-03-07T14:03:06Z
<p>Adr: </p>
<hr />
<div>* '''Description:''' acetyl-CoA carboxylase (beta subunit) <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''accD''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yttI ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || acetyl-CoA carboxylase (beta subunit))<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || production of malonyl-CoA, the substrate for fatty acid biosynthesis <br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 5.344 <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 786 bp, 262 aa <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[accA]]'', ''[[ytsJ]]''<br />
|-<br />
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14881&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''<br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:accD_context.gif]]<br />
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div><br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br/><br/><br/><br/><br />
<br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:''' BSU29210<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/accDA.html]<br />
<br />
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13926]<br />
<br />
=== Additional information===<br />
<br />
<br />
=The protein=<br />
<br />
=== Basic information/ Evolution ===<br />
<br />
* '''Catalyzed reaction/ biological activity:''' <br />
<br />
* '''Protein family:'''<br />
<br />
* '''Paralogous protein(s):'''<br />
<br />
=== Extended information on the protein ===<br />
<br />
* '''Kinetic information:''' <br />
<br />
* '''Domains:''' <br />
<br />
* '''Modification:'''<br />
<br />
* '''Cofactor(s):'''<br />
<br />
* '''Effectors of protein activity:'''<br />
<br />
* '''Interactions:''' ([[AccA]]-[[AccD]])-[[AccB]]-[[AccC]] (acetyl-CoA carboxylase complex)<br />
<br />
* '''Localization:''' Membrane-proximal (Spotty) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] Membrane-proximal (Spotty) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]<br />
<br />
=== Database entries ===<br />
<br />
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OD2 1OD2] (carboxytransferase from yeast, equivalent to [[AccA]]-[[AccD]]) {{PubMed|12663926}}<br />
<br />
* '''UniProt:''' [http://www.uniprot.org/uniprot/C0SP93 C0SP93]<br />
<br />
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29210]<br />
<br />
* '''E.C. number:''' [http://www.expasy.org/enzyme/6.4.1.2 6.4.1.2]<br />
<br />
=== Additional information===<br />
<br />
=Expression and regulation=<br />
* '''Operon:''' ''[[accD]]-[[accA]]'' (according to [http://dbtbs.hgc.jp/COG/prom/accDA.html DBTBS])<br />
<br />
* '''[[Sigma factor]]:''' <br />
<br />
* '''Regulation:''' <br />
** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]<br />
<br />
* '''Regulatory mechanism:''' <br />
** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutant:'''<br />
<br />
* '''Expression vector:'''<br />
<br />
* '''lacZ fusion:'''<br />
<br />
* '''GFP fusion:'''<br />
<br />
* '''two-hybrid system:''' <br />
<br />
* '''Antibody:'''<br />
<br />
=Labs working on this gene/protein=<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
==Reviews==<br />
<pubmed> 15952903 </pubmed><br />
==Original Publications==<br />
<pubmed>15066985, 12663926,14651647,16479537</pubmed><br />
<br />
[[Category:Protein-coding genes]]</div>
Adr