Difference between revisions of "Zwf"
| Line 39: | Line 39: | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
| − | + | <br/><br/> | |
| − | |||
| − | |||
| − | |||
| − | |||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| Line 66: | Line 62: | ||
=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| − | |||
=The protein= | =The protein= | ||
| Line 82: | Line 75: | ||
=== Extended information on the protein === | === Extended information on the protein === | ||
| − | * '''Kinetic information:''' Two Substrate Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/6292660 PubMed] | + | * '''Kinetic information:''' |
| + | ** Two Substrate Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/6292660 PubMed] | ||
| + | ** specific activity: 0.375 µmol min<sup>-1</sup> (mg protein)<sup>-1</sup> {{PubMed|24571712}} | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' Mg2+, Mn2+, Ca2+ |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
** NAD+, NADP+ and NADPH affect the enzyme kinetic [http://www.ncbi.nlm.nih.gov/pubmed/6292660 PubMed] | ** NAD+, NADP+ and NADPH affect the enzyme kinetic [http://www.ncbi.nlm.nih.gov/pubmed/6292660 PubMed] | ||
| − | * '''Interactions:''' | + | * '''[[SubtInteract|Interactions]]:''' |
| − | * '''Localization:''' | + | * '''[[Localization]]:''' |
=== Database entries === | === Database entries === | ||
| Line 116: | Line 111: | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=zwf_2479156_2480625_1 zwf] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=zwf_2479156_2480625_1 zwf] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' constitutive [http://www.ncbi.nlm.nih.gov/sites/entrez/12850135 PubMed] | * '''Regulation:''' constitutive [http://www.ncbi.nlm.nih.gov/sites/entrez/12850135 PubMed] | ||
| Line 147: | Line 142: | ||
=References= | =References= | ||
| − | <pubmed>6292660,12850135,19779711,7881907 </pubmed> | + | <pubmed>6292660,12850135,19779711,7881907 24571712</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:32, 28 February 2014
- Description: glucose 6-phosphate dehydrogenase, pentose-phosphate pathway
| Gene name | zwf |
| Synonyms | yqjJ |
| Essential | no |
| Product | glucose 6-phosphate dehydrogenase |
| Function | initiation of the pentose phosphate pathway |
| Gene expression levels in SubtiExpress: zwf | |
| Metabolic function and regulation of this protein in SubtiPathways: zwf | |
| MW, pI | 55 kDa, 5.28 |
| Gene length, protein length | 1467 bp, 489 aa |
| Immediate neighbours | rnz, gndA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU23850
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH (according to Swiss-Prot)
- Protein family: glucose-6-phosphate dehydrogenase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Cofactors: Mg2+, Mn2+, Ca2+
- Effectors of protein activity:
- NAD+, NADP+ and NADPH affect the enzyme kinetic PubMed
Database entries
- UniProt: P54547
- KEGG entry: [3]
- E.C. number: 1.1.1.49
Additional information
The enzyme is a dimer PubMed
Expression and regulation
- Operon: zwf PubMed
- Regulation: constitutive PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- SM-NB3 (zwf-spc), available in Anne Galinier's and Boris Görke's labs
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
