Difference between revisions of "Zwf"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 2681 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 3601 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:29, 17 April 2014

  • Description: glucose 6-phosphate dehydrogenase, pentose-phosphate pathway

Gene name zwf
Synonyms yqjJ
Essential no
Product glucose 6-phosphate dehydrogenase
Function initiation of the pentose phosphate pathway
Gene expression levels in SubtiExpress: zwf
Metabolic function and regulation of this protein in SubtiPathways:
zwf
MW, pI 55 kDa, 5.28
Gene length, protein length 1467 bp, 489 aa
Immediate neighbours rnz, gndA
Sequences Protein DNA DNA_with_flanks
Genetic context
Zwf context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Zwf expression.png















Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU23850

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH (according to Swiss-Prot)
  • Protein family: glucose-6-phosphate dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
    • Two Substrate Michaelis-Menten PubMed
    • specific activity: 0.375 µmol min-1 (mg protein)-1 PubMed
  • Modification:
  • Effectors of protein activity:
    • NAD+, NADP+ and NADPH affect the enzyme kinetic PubMed

Database entries

  • Structure: 1DPG (from Leuconostoc Mesenteroides, 45% identity, 62% similarity) PubMed
  • KEGG entry: [3]

Additional information

The enzyme is a dimer PubMed

Expression and regulation

  • Regulation: constitutive PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 2681 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 3601 PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:
  • FLAG-tag construct: GP1401 (spc, based on pGP1331), available in the Stülke lab

Labs working on this gene/protein

Your additional remarks

References