Difference between revisions of "Zwf"

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|style="background:#ABCDEF;" align="center"|'''Function''' || initiation of the pentose phosphate pathway  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || initiation of the pentose phosphate pathway  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU23850 zwf]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23850 zwf]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnz]]'', ''[[gndA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnz]]'', ''[[gndA]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14317&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23850 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23850 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23850 Advanced_DNA]
 
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Revision as of 13:13, 13 May 2013

  • Description: glucose 6-phosphate dehydrogenase, pentose-phosphate pathway

Gene name zwf
Synonyms yqjJ
Essential no
Product glucose 6-phosphate dehydrogenase
Function initiation of the pentose phosphate pathway
Gene expression levels in SubtiExpress: zwf
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 55 kDa, 5.28
Gene length, protein length 1467 bp, 489 aa
Immediate neighbours rnz, gndA
Sequences Protein DNA Advanced_DNA
Genetic context
Zwf context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Zwf expression.png




























Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU23850

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH (according to Swiss-Prot)
  • Protein family: glucose-6-phosphate dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: Two Substrate Michaelis-Menten PubMed
  • Domains:
  • Modification:
  • Cofactor(s): Mg2+, Mn2+, Ca2+
  • Effectors of protein activity:
    • NAD+, NADP+ and NADPH affect the enzyme kinetic PubMed
  • Interactions:
  • Localization:

Database entries

  • Structure: 1DPG (from Leuconostoc Mesenteroides, 45% identity, 62% similarity) PubMed
  • KEGG entry: [3]

Additional information

The enzyme is a dimer PubMed

Expression and regulation

  • Sigma factor:
  • Regulation: constitutive PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:
  • FLAG-tag construct: GP1401 (spc, based on pGP1331), available in the Stülke lab

Labs working on this gene/protein

Your additional remarks

References

Yun Xia Duan, Tao Chen, Xun Chen, Xue Ming Zhao
Overexpression of glucose-6-phosphate dehydrogenase enhances riboflavin production in Bacillus subtilis.
Appl Microbiol Biotechnol: 2010, 85(6);1907-14
[PubMed:19779711] [WorldCat.org] [DOI] (I p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

P Rowland, A K Basak, S Gover, H R Levy, M J Adams
The three-dimensional structure of glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides refined at 2.0 A resolution.
Structure: 1994, 2(11);1073-87
[PubMed:7881907] [WorldCat.org] [DOI] (P p)

S Ujita, K Kimura
Glucose-6-phosphate dehydrogenase, vegetative and spore Bacillus subtilis.
Methods Enzymol: 1982, 89 Pt D;258-61
[PubMed:6292660] [WorldCat.org] [DOI] (P p)