YydF

• Description: secreted peptide, controls LiaRS activity
  
  

Gene name	yydF
Synonyms
Essential	no
Product	secreted peptide
Function	control of LiaRS activity
MW, pI	5 kDa, 9.567
Gene length, protein length	147 bp, 49 aa
Immediate neighbours	yydG, fbp
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU40180

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: Q45596

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yydF-yydG-yydH-yydI-yydJ PubMed

• Sigma factor: SigA PubMed

• Regulation: activated after transition to stationary phase(AbrB) PubMed

• Regulatory mechanism: AbrB: transcription repression PubMed

• Additional information:
  • the mRNA is very stable (half-life > 15 min) PubMed
  • the yydF portion of the mRNA belongs to the most stable mRNAs in B. subtilis PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Bronwyn G Butcher, Yi-Pin Lin, John D Helmann
The yydFGHIJ operon of Bacillus subtilis encodes a peptide that induces the LiaRS two-component system.
J Bacteriol: 2007, 189(23);8616-25
[PubMed:17921301] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)