Difference between revisions of "YyaF"

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= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[GTP-binding proteins]]}},
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{{SubtiWiki category|[[proteins of unknown function]]}}
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= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[ComK regulon]]}}
  
 
=The gene=
 
=The gene=
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= Categories containing this gene/protein =
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{{SubtiWiki category|[[GTP-binding proteins]]}},
 
{{SubtiWiki category|[[proteins of unknown function]]}}
 
 
=The protein=
 
=The protein=
  

Revision as of 01:22, 9 December 2010

  • Description: GTP-binding protein/ GTPase

Gene name yyaF
Synonyms
Essential no
Product GTP-binding protein/ GTPase
Function unknown
MW, pI 39 kDa, 4.659
Gene length, protein length 1098 bp, 366 aa
Immediate neighbours rpsF, yyaE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YyaF context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

GTP-binding proteins, proteins of unknown function

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

  • Locus tag: BSU40920

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: EngD subfamily (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
    • ComK: transcription activation PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP836, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP844, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

Cordula Lindner, Reindert Nijland, Mariska van Hartskamp, Sierd Bron, Leendert W Hamoen, Oscar P Kuipers
Differential expression of two paralogous genes of Bacillus subtilis encoding single-stranded DNA binding protein.
J Bacteriol: 2004, 186(4);1097-105
[PubMed:14762004] [WorldCat.org] [DOI] (P p)

Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)