yyaF

yyaF
168

GTP-binding protein/ GTPase

locus
BSU_40920
Molecular weight
39.91 kDa
pI
4.66
Protein length
Gene length
function
unknown
product
GTP-binding protein/ GTPase
essential
no
synonyms
yyaF, engD

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0012 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,199,843 → 4,200,943
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The protein
Catalyzed reaction/ biological activity
Binds and hydrolyzes GTP and readily exchanges GDP for GTP
Protein family
TRAFAC class OBG-HflX-like GTPase superfamily (with [protein|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg] and [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA], according to UniProt)
[wiki|Domains]
OBG-typeG domain (aa 3-259) (according to UniProt)
[wiki|TGS domain] (aa 281-364) (according to UniProt)
Structure
[PDB|1JAL] (from Haemophilus influenzae, 58% identity) [pubmed|12837776]
[AF|P37518]
Paralogous protein(s)
[protein|CB68B3393CA7862C45C624AF7B1ADD188243A01F|era], [protein|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|ysxC], [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA], [protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF], [protein|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg], [protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH], [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA], [protein|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|engA]
Expression and Regulation
Operons
genes
[gene|BD162A17E5F3F9AB49E478CE72D0FE92D82E6673|yyzM]-[gene|FEE1952DFA3D6A5E2DC108B3B203B526642E2C68|yyaE]-[gene|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]-[gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]-[gene|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]
description
[Pubmed|22383849]
Open in new tab

[gene|BD162A17E5F3F9AB49E478CE72D0FE92D82E6673|yyzM]→[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]

2024-03-10 09:00:35

Jstuelk

88

9d6f6c8f552e3b902331ccb27a1c555fb109c0c2

9ABCA2D017C483FB4103C07C6AB9E7C773168C9B

genes
[gene|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]-[gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]-[gene|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]
description
[Pubmed|14762004]
regulation
[protein|search|ComK]: transcription activation [Pubmed|11948146]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|24310371], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]→[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]

2024-03-18 10:19:01

ghost

90

919e120921039bf82b5f231ffb9cdc8bf1c3e019

AB203DF546C89702CE168BF3D045E792086CFD74

Biological materials
Mutant
MGNA-B869 (yyaF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1868 NBRP B. subtilis, Japan]
BKE40920 (Δ[gene|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTTTCATCTCCTCTAT,  downstream forward: _UP4_TAGGATGCAGTTGTAAAGGG
BKK40920 (Δ[gene|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTTTCATCTCCTCTAT,  downstream forward: _UP4_TAGGATGCAGTTGTAAAGGG
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP836, available in [wiki|Stülke] lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP844, available in [wiki|Stülke] lab
labs
[wiki|Naotake Ogasawara], Nara, Japan
References
Reviews
21885683
Original Publications
12427945,14762004,11948165,11948146,12837776

C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73

Page visits: 2514

Time of last update: 2024-03-28 17:49:03

Author of last update: Melvin.boenninger