yyaF

168

GTP-binding protein/ GTPase

locus: BSU_40920

Molecular weight: 39.91 kDa

pI: 4.66

Protein length: 366 aa

Gene length: 1101 bp

function: unknown

product: GTP-binding protein/ GTPase

essential: no

synonyms: yyaF, engD

List of homologs in different organisms, belongs to COG0012 (Galperin et al., 2021)

Gene

Coordinates: 4,199,843 → 4,200,943

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The protein

Catalyzed reaction/ biological activity

• Binds and hydrolyzes GTP and readily exchanges GDP for GTP

Protein family

• TRAFAC class OBG-HflX-like GTPase superfamily (with [protein|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg] and [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA], according to UniProt)

[wiki|Domains]

• OBG-typeG domain (aa 3-259) (according to UniProt)
• [wiki|TGS domain] (aa 281-364) (according to UniProt)

Structure

• [PDB|1JAL] (from Haemophilus influenzae, 58% identity) [pubmed|12837776]
• [AF|P37518]

Paralogous protein(s)

• [protein|CB68B3393CA7862C45C624AF7B1ADD188243A01F|era], [protein|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|ysxC], [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA], [protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF], [protein|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg], [protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH], [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA], [protein|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|engA]

Biological materials

Mutant

• MGNA-B869 (yyaF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1868 NBRP B. subtilis, Japan]
• BKE40920 (Δ[gene|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTTTCATCTCCTCTAT,  downstream forward: _UP4_TAGGATGCAGTTGTAAAGGG
• BKK40920 (Δ[gene|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTTTCATCTCCTCTAT,  downstream forward: _UP4_TAGGATGCAGTTGTAAAGGG

Expression vectors

• for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP836, available in [wiki|Stülke] lab
• for expression/ purification from ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP844, available in [wiki|Stülke] lab

labs

• [wiki|Naotake Ogasawara], Nara, Japan

References

Reviews

• 21885683

Original Publications

• 12427945,14762004,11948165,11948146,12837776