wapI

168

immunity protein, protects the cell against the toxic activity of WapA

locus: BSU_39220

Molecular weight: 16.29 kDa

pI: 4.42

Protein length: 142 aa

Gene length: 429 bp

function: intercellular competition

product: immunity protein

essential: yes

synonyms: wapI, yxxG

List of homologs in different organisms

Gene

Coordinates: 4,023,054 → 4,023,482

Phenotypes of a mutant

• essential [Pubmed|28189581]
• severe growth defect  [Pubmed|20815827]

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The protein

Catalyzed reaction/ biological activity

• [protein|C7400733DB8835A204A7FF26C87A92A15C5F318C|wapI] inhibits the toxic activity of [protein|BBC06DA57CAC2B2378763E0839C6C31AFBEA0417|wapA] [Pubmed|23572593]

Structure

• [AF|Q07836]

Modification

• phosphorylation on (Tyr-102 OR Ser-105) [Pubmed|17218307]

Biological materials

Mutant

• MGNA-B789 (yxxG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1788 NBRP B. subtilis, Japan]
• BKE39220 (Δ[gene|C7400733DB8835A204A7FF26C87A92A15C5F318C|wapI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTTCCTCCTCATTTAA,  downstream forward: _UP4_TAATTATAAATAATTTCTGC
• BKK39220 (Δ[gene|C7400733DB8835A204A7FF26C87A92A15C5F318C|wapI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTTCCTCCTCATTTAA,  downstream forward: _UP4_TAATTATAAATAATTTCTGC

References

• 16672620,20815827,17218307,16306698,9537385,23572593,23199363,26923784,28189581