hbuT

168

sporulation protein, transporter for 3-hydroxybutyrate

locus: BSU_39000

Molecular weight: 49.98 kDa

pI: 8.9

Protein length: 472 aa

Gene length: 1419 bp

function: 3-hydroxybutyrate utilization

product: transporter for 3-hydroxybutyrate

essential: no

synonyms: hbuT, yxjC

List of homologs in different organisms, belongs to COG2610 (Galperin et al., 2021)

Gene

Coordinates: 4,002,716 → 4,004,134

Phenotypes of a mutant

• reduced uptake of 3-hydroxybutyrate during [wiki|sporulation] [Pubmed|26363016]

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The protein

Catalyzed reaction/ biological activity

• uptake of 3-hydroxybutyrate [Pubmed|26363016]

Protein family

• [wiki|CitM transporter family (TC 2.A.11)] (according to UniProt)

Structure

• [AF|P42314]

[wiki|Localization]

• cell membrane (according to UniProt)

Biological materials

Mutant

• MGNA-B727 (yxjC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1726 NBRP B. subtilis, Japan]
• BKE39000 (Δ[gene|B416BB368A96926526458B2258BF8AB928D7C15E|hbuT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39000 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACACACACATCCCCCGTTT,  downstream forward: _UP4_TAGAAGAAAACGAAAGGGGC
• BKK39000 (Δ[gene|B416BB368A96926526458B2258BF8AB928D7C15E|hbuT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39000 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACACACACATCCCCCGTTT,  downstream forward: _UP4_TAGAAGAAAACGAAAGGGGC

References

• 16672620,10746760,12662922,12850135,10666464,26363016