YxeN

• Description: putative cysteine ABC transporter (permease), the yxeK-snaB-yxeM-yxeN-yxeO-sndB-yxeQ operon may be implicated in the utilization of a modified cysteine variant
  
  

Gene name	yxeN
Synonyms
Essential	no
Product	putative cysteine ABC transporter (permease)
Function	uptake of cysteine
Gene expression levels in SubtiExpress: yxeN
Interactions involving this protein in SubtInteract: YxeN
MW, pI	24 kDa, 9.484
Gene length, protein length	672 bp, 224 aa
Immediate neighbours	yxeO, yxeM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of amino acids, membrane proteins

This gene is a member of the following regulons

CymR regulon

The gene

Basic information

• Locus tag: BSU39490

Database entries

• BsubCyc: BSU39490

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: binding-protein-dependent transport system permease family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: YxeO-YxeN-YxeM PubMed

• Localization: cell membrane PubMed

Database entries

• BsubCyc: BSU39490

• Structure:

• UniProt: P54953

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yxeK-snaB-yxeM-yxeN-yxeO-sndB-yxeQ PubMed

• Expression browser: yxeN PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of cysteine (CymR) PubMed

• Regulatory mechanism:
  • CymR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A950 ( yxeN::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Sergine Even, Pierre Burguière, Sandrine Auger, Olga Soutourina, Antoine Danchin, Isabelle Martin-Verstraete
Global control of cysteine metabolism by CymR in Bacillus subtilis.
J Bacteriol: 2006, 188(6);2184-97
[PubMed:16513748] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)