yxeK

yxeK
168

N-acetyl S-(2-succino)cysteine oxygenase, detoxification of S-(2-succino)-adducts via oxygenolytic C-S bond cleavage

locus
BSU_39520
Molecular weight
49.18 kDa
pI
5.67
Protein length
Gene length
function
utilization and detoxification of S-(2-succino)cysteine
product
N-acetyl S-(2-succino)cysteine oxygenase
essential
no
synonyms
yxeK

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2141 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,060,818  4,062,143
Phenotypes of a mutant
no growth with S-(2-succino)cysteine as the single source of sulfur [pubmed|29626092]
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The protein
Catalyzed reaction/ biological activity
oxygenation of N-acetyl S-(2-succino)cysteine, resulting in C-S bond cleavage (N-acetylcysteine + oxaloacetate) [pubmed|34510734,29626092]
Protein family
ntaA/snaA/soxA(dszA) monooxygenase family (with [protein|8145952307BC47805A7A9BD39418474358043BFE|cmoJ], according to UniProt)
[wiki|Cofactors]
FMN [pubmed|34510734]
Structure
[PDB|5TLC] (from B. subtilis WU-S2B 42% identity) [pubmed|28205250]
[AF|P54950]
Paralogous protein(s)
[protein|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]
Expression and Regulation
Operons
genes
[gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]-[gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]-[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]-[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]-[gene|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO]-[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]-[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]
description
[Pubmed|10746760]
regulation
repressed in the presence of cysteine ([protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]) [Pubmed|16513748]
regulatory mechanism
[protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16513748], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16513748], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the amount of the mRNA is substantially decreased upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
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[gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]→[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]

2024-02-23 23:35:50

Bzhu

62

26e7d8a77b7f64e210508bce49d61eed26cdd5aa

11F447FD5833B59A1639842D70685F9A6B369EAF

Biological materials
Mutant
MGNA-B709 (yxeK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1708 NBRP B. subtilis, Japan]
BKE39520 ([gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39520 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGCGATACTCCTTTTAT,  downstream forward: _UP4_TATGCAAAGTAAGGAGGAAC
BKK39520 ([gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39520 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGCGATACTCCTTTTAT,  downstream forward: _UP4_TATGCAAAGTAAGGAGGAAC
References
10746760,16513748,21815947,28205250,29497411,29626092,34510734,36309089

19E6EF9CCA31440EAC046F8D6CA98B6A08589D58

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Time of last update: 2024-03-28 20:10:40

Author of last update: Jstuelk