yxaB

168

general stress protein, similar to pyruvyl transferase

locus: BSU_40030

Molecular weight: 39.61 kDa

pI: 4.53

Protein length: 343 aa

Gene length: 1032 bp

function: biofilm formation, survival of salt and ethanol stress

product: putative exopolysaccaride synthase

essential: no

synonyms: yxaB

List of homologs in different organisms, belongs to COG5039 (Galperin et al., 2021)

Gene

Coordinates: 4,110,949  4,111,980

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The protein

Protein family

• polysaccharide pyruvyl transferase family (with [protein|6EBD8F7065B6C1996067F3120DD9CFEA91D3A444|epsI] and [protein|52A47B49B1FB955EE7E3C316B57A2B4134F78480|epsO], according to UniProt)

Structure

• [PDB|5AX7] (from yeast, 30% identity) [pubmed|27194449]
• [AF|P42101]

Paralogous protein(s)

• [protein|6EBD8F7065B6C1996067F3120DD9CFEA91D3A444|epsI]

Biological materials

Mutant

• MGNA-B681 (yxaB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1680 NBRP B. subtilis, Japan]
• BKE40030 ([gene|9A223AE85131511B7EA3A71EA57D87E8B167FA67|yxaB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCTGCGTCCTCCTTCT,  downstream forward: _UP4_TAAACGAAAAAGACTGCCCT
• BKK40030 ([gene|9A223AE85131511B7EA3A71EA57D87E8B167FA67|yxaB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCTGCGTCCTCCTTCT,  downstream forward: _UP4_TAAACGAAAAAGACTGCCCT

References

• 10746760,15805528,27194449