YwlF

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  • Description: ribose-5-phosphate isomerase

Gene name ywlF
Synonyms ipc-32d
Essential no
Product ribose-5-phosphate isomerase
Function pentose phosphate pathway
Gene expression levels in SubtiExpress: ywlF
Metabolic function and regulation of this protein in SubtiPathways:
ywlF
MW, pI 16 kDa, 5.416
Gene length, protein length 447 bp, 149 aa
Immediate neighbours ywlG, ywlE
Sequences Protein DNA DNA_with_flanks
Genetic context
YwlF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YwlF expression.png




























Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

  • Locus tag: BSU36920

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: lacAB/rpiB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: Reversible Mass Action Kinetics PubMed PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • Inhibited by 6-phosphogluconate and AMP PubMed
    • Inhibited by divalent ions such as Ag2+, Mg2+, Co2+ and Zn2+ PubMed
    • Activated by EDTA and 2-mercaptoethanol PubMed
  • Interactions:
  • Localization:

Database entries

  • Structure: 3HEE (from Clostridium thermocellum, 55% identity, 72% similarity)
  • KEGG entry: [3]

Additional information

The enzyme has probably more than one active site, but with no cooperativity described PubMed

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 367 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1467 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:
  • FLAG-tag construct: GP1404 (spc, based on pGP1331), available in the Stülke lab

Labs working on this gene/protein

Your additional remarks

References

Shuobo Shi, Tao Chen, Zhigang Zhang, Xun Chen, Xueming Zhao
Transcriptome analysis guided metabolic engineering of Bacillus subtilis for riboflavin production.
Metab Eng: 2009, 11(4-5);243-52
[PubMed:19446032] [WorldCat.org] [DOI] (I p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

R D MacElroy, C R Middaugh
Bacterial ribosephosphate isomerase.
Methods Enzymol: 1982, 89 Pt D;571-9
[PubMed:7144591] [WorldCat.org] [DOI] (P p)

C R Middaugh, R D MacElroy
The effect of temperature on ribose-5-phosphate isomerase from a mesophile, Thiobacillus thioparus, and a thermophile, Bacillus caldolyticus.
J Biochem: 1976, 79(6);1331-44
[PubMed:956158] [WorldCat.org] [DOI] (P p)