Difference between revisions of "YwlF"

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(Biological materials)
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* '''Antibody:'''
 
* '''Antibody:'''
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* '''FLAG-tag construct:''' GP1404 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=

Revision as of 13:58, 22 February 2011

  • Description: ribose-5-phosphate isomerase

Gene name ywlF
Synonyms ipc-32d
Essential no
Product ribose-5-phosphate isomerase
Function pentose phosphate pathway
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 16 kDa, 5.416
Gene length, protein length 447 bp, 149 aa
Immediate neighbours ywlG, ywlE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwlF context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

  • Locus tag: BSU36920

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: lacAB/rpiB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: Reversible Mass Action Kinetics PubMed PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • Inhibited by 6-phosphogluconate and AMP PubMed
    • Inhibited by divalent ions such as Ag2+, Mg2+, Co2+ and Zn2+ PubMed
    • Activated by EDTA and 2-mercaptoethanol PubMed
  • Interactions:
  • Localization:

Database entries

  • Structure: 3HEE (from Clostridium thermocellum, 55% identity, 72% similarity)
  • KEGG entry: [3]

Additional information

The enzyme has probably more than one active site, but with no cooperativity described PubMed

Expression and regulation

  • Regulation:
    • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:
  • FLAG-tag construct: GP1404 (spc, based on pGP1331), available in the Stülke lab

Labs working on this gene/protein

Your additional remarks

References