Difference between revisions of "YwlF"

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(Database entries)
(References)
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<pubmed> 19446032 </pubmed>
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<pubmed> 19446032 956158 7144591 </pubmed>
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 13:59, 10 June 2009

  • Description: ribose-5-phosphate isomerase

Gene name ywlF
Synonyms ipc-32d
Essential no
Product ribose-5-phosphate isomerase
Function pentose phosphate pathway
MW, pI 16 kDa, 5.416
Gene length, protein length 447 bp, 149 aa
Immediate neighbours ywlG, ywlE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwlF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU36920

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: lacAB/rpiB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: Reversible Mass Action Kinetics PubMed PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • Inhibited by 6-phosphogluconate and AMP PubMed
    • Inhibited by divalent ions such as Ag2+, Mg2+, Co2+ and Zn2+ PubMed
    • Activated by EDTA and 2-mercaptoethanol PubMed
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

The enzyme has probably more than one active site, but with no cooperativity described PubMed

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Shuobo Shi, Tao Chen, Zhigang Zhang, Xun Chen, Xueming Zhao
Transcriptome analysis guided metabolic engineering of Bacillus subtilis for riboflavin production.
Metab Eng: 2009, 11(4-5);243-52
[PubMed:19446032] [WorldCat.org] [DOI] (I p)

R D MacElroy, C R Middaugh
Bacterial ribosephosphate isomerase.
Methods Enzymol: 1982, 89 Pt D;571-9
[PubMed:7144591] [WorldCat.org] [DOI] (P p)

C R Middaugh, R D MacElroy
The effect of temperature on ribose-5-phosphate isomerase from a mesophile, Thiobacillus thioparus, and a thermophile, Bacillus caldolyticus.
J Biochem: 1976, 79(6);1331-44
[PubMed:956158] [WorldCat.org] [DOI] (P p)

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed