Difference between revisions of "YwlE"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36930&redirect=T BSU36930] | ||
* '''DBTBS entry:''' no entry | * '''DBTBS entry:''' no entry | ||
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36930&redirect=T BSU36930] | ||
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1ZGG 1ZGG] | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1ZGG 1ZGG] | ||
Revision as of 15:02, 2 April 2014
- Description: protein arginine phosphatase
| Gene name | ywlE |
| Synonyms | ipc-31d |
| Essential | no |
| Product | protein arginine phosphatase |
| Function | dephosphorylation of McsB |
| Gene expression levels in SubtiExpress: ywlE | |
| MW, pI | 16 kDa, 6.681 |
| Gene length, protein length | 450 bp, 150 aa |
| Immediate neighbours | ywlF, ywlD |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU36930
Phenotypes of a mutant
Database entries
- BsubCyc: BSU36930
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Protein arginine phosphate + H2O = protein arginine + phosphate PubMed
- Protein family:
- Paralogous protein(s): YfkJ
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization: weak oligomerization, results in inactivation of YwlE PubMed
Database entries
- BsubCyc: BSU36930
- Structure: 1ZGG
- UniProt: P39155
- KEGG entry: [2]
- E.C. number: 3.1.3.48
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- ywlE::aphA3 available from Ulf Gerth's lab
- ywlE::tet available from Ulf Gerth's lab
- ywlE::spec available from Ulf Gerth's lab
- GP1458 (ywlE::aphA3), available in Jörg Stülke's lab
- GP1459 (ywlE::tet), available in Jörg Stülke's lab
- Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody: available in Ulf Gerth's lab
Labs working on this gene/protein
- Ulf Gerth, Greifswald, Germany
- Fabian Commichau Göttingen, Germany
Your additional remarks
References
