ywlE
168
protein arginine phosphatase
locus
BSU_36930
Molecular weight
16.64 kDa
pI
6.68
function
dephosphorylation of [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]
product
protein arginine phosphatase
essential
no
ec
3.1.3.48
synonyms
ywlE, ipc-31d
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0394 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,791,805 3,792,257
Phenotypes of a mutant
impaired spore germination [pubmed|31221751]
The protein
Catalyzed reaction/ biological activity
Protein arginine phosphate + H2O --> protein arginine + phosphate [Pubmed|22517742]
Protein family
low molecular weight phosphotyrosine protein phosphatase family (with [protein|DB3527FB78D0B7C144D1699D665788184F19AFE0|arsC] and [protein|2CEFD8D9692D062CC09E3DEDA1A4015C76BC4167|yfkJ], according to UniProt)
Structure
[PDB|1ZGG]
[AF|P39155]
Effectors of protein activity
activity is inhibited by oxidative stress [Pubmed|27524296]
Paralogous protein(s)
[protein|2CEFD8D9692D062CC09E3DEDA1A4015C76BC4167|yfkJ]
[wiki|Localization]
weak oligomerization, results in inactivation of YwlE [Pubmed|19678837]
Expression and Regulation
Operons
genes
[gene|19829F6D993188BE854F0F61CB93777AD04CF754|ywlD]-[gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]-[gene|91827FEC70F5EEB8B072D06ABA05C19FC54D5F71|ywlF]-[gene|41407DE15D91959DA92799623F1364811C170F35|ywlG]
description
[Pubmed|22383849]
genes
[gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]-[gene|91827FEC70F5EEB8B072D06ABA05C19FC54D5F71|ywlF]-[gene|41407DE15D91959DA92799623F1364811C170F35|ywlG]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A186 (ywlE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/186 NBRP B. subtilis, Japan]
''ywlE::aphA3'' available from [wiki|Ulf Gerth]'s lab
''ywlE::tet'' available from [wiki|Ulf Gerth]'s lab
''ywlE::spec'' available from [wiki|Ulf Gerth]'s lab
GP1458 (''ywlE''::''aphA3''), available in [wiki|Jörg Stülke]'s lab
GP1459 (''ywlE''::''tet''), available in [wiki|Jörg Stülke]'s and [wiki|Fabian Commichau]'s labs [Pubmed|25610436]
BKE36930 ([gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAGTCACCCCTTATT, downstream forward: _UP4_TAAGTTGTCAGAAAATCTGC
BKK36930 ([gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAGTCACCCCTTATT, downstream forward: _UP4_TAAGTTGTCAGAAAATCTGC
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [wiki|Ulf Gerth]'s lab
expression of native ''ywlE'' in ''B. subtilis'': pBP183 (in [wiki|pBQ200]), available in [wiki|Fabian Commichau]'s lab
Antibody
available in [wiki|Ulf Gerth]'s lab
labs
[wiki|Ulf Gerth], Greifswald, Germany
[wiki|Fabian Commichau] Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]
References
Reviews
Original Publications
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Time of last update: 2024-04-19 19:57:59
Author of last update: Jstuelk