Difference between revisions of "YwlE"

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* '''Description:''' [[protein kinases and phosphatases|protein tyrosine phosphatase]], [[protein kinases and phosphatases|protein arginine phosphatase]] <br/><br/>
+
* '''Description:''' [[protein kinases and phosphatases|protein arginine phosphatase]] <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[protein kinases and phosphatases|protein tyrosine phosphatase]], [[protein kinases and phosphatases|protein arginine phosphatase]]
+
|style="background:#ABCDEF;" align="center"| '''Product''' || [[protein kinases and phosphatases|protein arginine phosphatase]]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || dephosphorylation of [[McsB]]
 
|style="background:#ABCDEF;" align="center"|'''Function''' || dephosphorylation of [[McsB]]
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' Protein tyrosine phosphate + H<sub>2</sub>O = protein tyrosine + phosphate (according to Swiss-Prot)
+
* '''Catalyzed reaction/ biological activity:''' Protein arginine phosphate + H<sub>2</sub>O = protein arginine + phosphate {{PubMed|22517742}}
  
* '''Protein family:''' low molecular weight phosphotyrosine [[protein kinases and phosphatases|protein phosphatase]] family (according to Swiss-Prot)
+
* '''Protein family:'''  
  
 
* '''Paralogous protein(s):''' [[YfkJ]]
 
* '''Paralogous protein(s):''' [[YfkJ]]
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' ''ywlE::aphA3'' availbale from the Gerth lab
+
* '''Mutant:''' ''ywlE::aphA3'' available from [[Ulf Gerth]]'s lab
''ywlE::tet'' availbale from the Gerth lab
+
''ywlE::tet'' available from [[Ulf Gerth]]'s lab
''ywlE::spec'' availbale from the Gerth lab
+
''ywlE::spec'' available from [[Ulf Gerth]]'s lab
  
* '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
+
* '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [[Ulf Gerth]]'s lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
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* '''two-hybrid system:'''  
 
* '''two-hybrid system:'''  
  
* '''Antibody:''' available in Gerth lab
+
* '''Antibody:''' available in [[Ulf Gerth]]'s lab
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 
+
* [[Ulf Gerth]], Greifswald, Germany
 
=Your additional remarks=
 
=Your additional remarks=
  
 
=References=
 
=References=
  
<pubmed>16452434,15866923,15995210,19678837 21622759, 20852588 </pubmed>
+
<pubmed>16452434,15866923,15995210,19678837 21622759, 20852588 22517742</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 18:46, 21 April 2012

Gene name ywlE
Synonyms ipc-31d
Essential no
Product protein arginine phosphatase
Function dephosphorylation of McsB
MW, pI 16 kDa, 6.681
Gene length, protein length 450 bp, 150 aa
Immediate neighbours ywlF, ywlD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwlE context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

protein modification

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU36930

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein arginine phosphate + H2O = protein arginine + phosphate PubMed
  • Protein family:
  • Paralogous protein(s): YfkJ

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: ywlE::aphA3 available from Ulf Gerth's lab

ywlE::tet available from Ulf Gerth's lab ywlE::spec available from Ulf Gerth's lab

  • Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:

Labs working on this gene/protein

Your additional remarks

References

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

A K W Elsholz, K Hempel, S Michalik, K Gronau, D Becher, M Hecker, U Gerth
Activity control of the ClpC adaptor McsB in Bacillus subtilis.
J Bacteriol: 2011, 193(15);3887-93
[PubMed:21622759] [WorldCat.org] [DOI] (I p)

Alexander K W Elsholz, Stephan Michalik, Daniela Zühlke, Michael Hecker, Ulf Gerth
CtsR, the Gram-positive master regulator of protein quality control, feels the heat.
EMBO J: 2010, 29(21);3621-9
[PubMed:20852588] [WorldCat.org] [DOI] (I p)

Jascha Blobel, Pau Bernadó, Huimin Xu, Changwen Jin, Miquel Pons
Weak oligomerization of low-molecular-weight protein tyrosine phosphatase is conserved from mammals to bacteria.
FEBS J: 2009, 276(16);4346-57
[PubMed:19678837] [WorldCat.org] [DOI] (I p)

Huimin Xu, Bin Xia, Changwen Jin
Solution structure of a low-molecular-weight protein tyrosine phosphatase from Bacillus subtilis.
J Bacteriol: 2006, 188(4);1509-17
[PubMed:16452434] [WorldCat.org] [DOI] (P p)

Lucia Musumeci, Cristina Bongiorni, Lutz Tautz, Robert A Edwards, Andrei Osterman, Marta Perego, Tomas Mustelin, Nunzio Bottini
Low-molecular-weight protein tyrosine phosphatases of Bacillus subtilis.
J Bacteriol: 2005, 187(14);4945-56
[PubMed:15995210] [WorldCat.org] [DOI] (P p)

Ivan Mijakovic, Lucia Musumeci, Lutz Tautz, Dina Petranovic, Robert A Edwards, Peter Ruhdal Jensen, Tomas Mustelin, Josef Deutscher, Nunzio Bottini
In vitro characterization of the Bacillus subtilis protein tyrosine phosphatase YwqE.
J Bacteriol: 2005, 187(10);3384-90
[PubMed:15866923] [WorldCat.org] [DOI] (P p)