maeA

168

malic enzyme, required for malate dependent catabolite repression of the [gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]-[gene|F6C3B267106E7510FABECF0B13ABF222901689A9|pftB] operon

locus: BSU_37050

Molecular weight: 63.93 kDa

pI: 4.88

Protein length: 582 aa

Gene length: 1749 bp

function: malate utilization

product: NAD-dependent malate dehydrogenase

essential: no

ec: 1.1.1.38

synonyms: maeA, ywkA

List of homologs in different organisms, belongs to COG0281 (Galperin et al., 2021)

Gene

Coordinates: 3,800,418  3,802,166

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The protein

Catalyzed reaction/ biological activity

• (S)-malate + NAD⁺ --> CO₂ + NADH + pyruvate (according to UniProt)
• H⁺ + oxaloacetate --> CO₂ + pyruvate (according to UniProt)

Protein family

• [wiki|malic enzymes family] (according to UniProt)

[wiki|Cofactors]

• NAD⁺

Structure

• [PDB|1LLQ] (from Ascaris suum, 41% identity) [pubmed|12033925]
• [AF|P45868]

Paralogous protein(s)

• [protein|BBBCD5F56779895189E21076AF165B901F654534|malS]

Biological materials

Mutant

• MGNA-B657 (ywkA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1656 NBRP B. subtilis, Japan]
• GP1141 (aphA3) available in [wiki|Jörg Stülke]'s lab
• BKE37050 ([gene|4F30A4412768E6313F57BD56C4EF5079F500E0C4|maeA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC,  downstream forward: _UP4_TAATTAGCGAGTAAATTTTT
• BKK37050 ([gene|4F30A4412768E6313F57BD56C4EF5079F500E0C4|maeA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC,  downstream forward: _UP4_TAATTAGCGAGTAAATTTTT

labs

• [wiki|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

• 21815947,23136871,12949160,16788182,28974613,12033925