ywfM

168

unknown

locus: BSU_37630

Molecular weight: 31.19 kDa

pI: 9.77

Protein length: 296 aa

Gene length: 891 bp

function: unknown

product: unknown

essential: no

ec: null

synonyms: ywfM, ipa-91d

List of homologs in different organisms, belongs to COG5006 (Galperin et al., 2021)

Gene

Coordinates: 3,862,357  3,863,247

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The protein

Protein family

• [wiki|EamA transporter family] (according to UniProt)

[wiki|Domains]

• 2 [wiki|EamA domain]s (aa 15-138, aa 158-282) (according to UniProt)

Structure

• [AF|P39649]

[wiki|Localization]

• cell membrane (according to UniProt)

Biological materials

Mutant

• MGNA-A582 (ywfM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/582 NBRP B. subtilis, Japan]
• BKE37630 ([gene|522E49C265286CBC191334F4EFD864D81D59E3CB|ywfM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGCCTCTCCCCTTTAA,  downstream forward: _UP4_TAATAGATTCACATAAGCTT
• BKK37630 ([gene|522E49C265286CBC191334F4EFD864D81D59E3CB|ywfM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGCCTCTCCCCTTTAA,  downstream forward: _UP4_TAATAGATTCACATAAGCTT

Expression vectors

• pGP3737: expression of [gene|522E49C265286CBC191334F4EFD864D81D59E3CB|ywfM] (''gapA'' RBS) by [wiki|pBQ200] in ''B. subtilis'', available in [wiki|Jörg Stülke]'s lab

References

• 20525796