ilvK
168
branched-chain amino acid aminotransferase
locus
BSU_38550
Molecular weight
40.16 kDa
pI
4.95
function
biosynthesis of branched-chain amino acids
product
branched-chain amino acid aminotransferase
essential
no
ec
2.6.1.42
synonyms
ywaA, ipa-0r
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0115 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,957,391 3,958,482
The protein
Catalyzed reaction/ biological activity
2-oxoglutarate + L-leucine --> 4-methyl-2-oxopentanoate + L-glutamate (according to UniProt)
2-oxoglutarate + L-isoleucine --> (S)-3-methyl-2-oxopentanoate + L-glutamate (according to UniProt)
2-oxoglutarate + L-valine --> 3-methyl-2-oxobutanoate + L-glutamate (according to UniProt)
Protein family
[wiki|Class-IV pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Cofactors]
PLP
Structure
[PDB|3HT5] (from '' Mycobacterium tuberculosis'', 42% identity, 58% similarity) [Pubmed|19923721]
[AF|P39576]
Modification
S-cysteinylation after diamide stress (C104) [Pubmed|17611193]
Paralogous protein(s)
[protein|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ilvE]
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Biological materials
Mutant
MGNA-B757 (ywaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1756 NBRP B. subtilis, Japan]
a ''ywaA::spc'' mutant and a ''[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd] [gene|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ilvE] [gene|8163996FCB9D02F771E7A04A91D5720027260F12|ilvK]'' triple mutant are available in [wiki|Linc Sonenshein]'s lab
BKE38550 ([gene|8163996FCB9D02F771E7A04A91D5720027260F12|ilvK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTCAATCTCCCTGCTG, downstream forward: _UP4_TAAGAAAAAAGCCGGCCCAT
BKK38550 ([gene|8163996FCB9D02F771E7A04A91D5720027260F12|ilvK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTCAATCTCCCTGCTG, downstream forward: _UP4_TAAGAAAAAAGCCGGCCCAT
References
Page visits: 4742
Time of last update: 2024-04-24 06:01:27
Author of last update: Robert.warneke