yvrC

168

[wiki|ABC transporter] (binding protein) for cobalamin

locus: BSU_33180

Molecular weight: 34.08 kDa

pI: 4.93

Protein length: 314 aa

Gene length: 945 bp

function: uptake of cobalamin

product: [wiki|ABC transporter] (binding protein)

essential: no

synonyms: yvrC

List of homologs in different organisms, belongs to COG0614 (Galperin et al., 2021)

Gene

Coordinates: 3,403,493 → 3,404,437

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The protein

Protein family

• [wiki|Bacterial solute-binding protein 8 family] (according to UniProt)

[wiki|Domains]

• [wiki|Fe/B12 periplasmic-binding domain] (aa 59-311) (according to UniProt)

Structure

• [PDB|4M7O] (from Staphylococcus epidermidis, 45% identity)
• [AF|O34805]

[wiki|Localization]

• cell membrane (according to UniProt)

Biological materials

Mutant

• MGNA-B045 (yvrC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1044 NBRP B. subtilis, Japan]
• BKE33180 (Δ[gene|C76265F521D9A153F28AC6BACD935CC20DEA5D9C|yvrC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAATCTTTTCCTCCTAAA,  downstream forward: _UP4_CGTCTGATCGAAGGGGTCGA
• BKK33180 (Δ[gene|C76265F521D9A153F28AC6BACD935CC20DEA5D9C|yvrC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAATCTTTTCCTCCTAAA,  downstream forward: _UP4_CGTCTGATCGAAGGGGTCGA

References

• 10092453,22383849,14704351,32544228