yvdF

168

glucan 1,4-alpha-maltohydrolase, neopullulanase, maltogenic amylase

locus: BSU_34620

Molecular weight: 68.68 kDa

pI: 5.18

Protein length: 589 aa

Gene length: 1770 bp

function: starch and maltodextrin utilization

product: glucan 1,4-alpha-maltohydrolase, neopullulanase, maltogenic amylase

essential: no

synonyms: yvdF, bbmA

List of homologs in different organisms, belongs to COG0366 (Galperin et al., 2021)

Gene

Coordinates: 3,555,902  3,557,671

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The protein

Protein family

• [wiki|glycosyl hydrolase 13 family] (according to UniProt)

Structure

• [PDB|1J0J] (from G. stearothermophilus, 56% identity, complex with maltotetraose),  [PDB|1J0H] (from G. stearothermophilus, 56% identity)
• [AF|O06988]

[wiki|Localization]

• on both sides of the cytoplasmic membrane and in the periplasm during vegetative growth but in the cytoplasm of prespores [Pubmed|19465663]

Biological materials

Mutant

• MGNA-B628 (yvdF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1627 NBRP B. subtilis, Japan]
• BKE34620 ([gene|A577441DE4ABAF624D507DB80FBED2A652BCF385|yvdF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCCCTTTGATTT,  downstream forward: _UP4_TAACATTCTGTTATCGGTAA
• BKK34620 ([gene|A577441DE4ABAF624D507DB80FBED2A652BCF385|yvdF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCCCTTTGATTT,  downstream forward: _UP4_TAACATTCTGTTATCGGTAA

References

• 16707683,19465663,15650689