Difference between revisions of "YvcK"
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|style="background:#ABCDEF;" align="center"|'''Function''' || localization of [[ponA|PBP1]] | |style="background:#ABCDEF;" align="center"|'''Function''' || localization of [[ponA|PBP1]] | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34760 yvcK] |
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 5.408 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 5.408 | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvcL]]'', ''[[yvcJ]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvcL]]'', ''[[yvcJ]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34760 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34760 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34760 Advanced_DNA] |
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|colspan="2" | '''Genetic context''' <br/> [[Image:yvcK_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:yvcK_context.gif]] | ||
Revision as of 13:56, 13 May 2013
- Description: essential for growth under gluconeogenetic conditions, required for correct localization of PBP1
| Gene name | yvcK |
| Synonyms | |
| Essential | no |
| Product | unknown |
| Function | localization of PBP1 |
| Gene expression levels in SubtiExpress: yvcK | |
| MW, pI | 34 kDa, 5.408 |
| Gene length, protein length | 951 bp, 317 aa |
| Immediate neighbours | yvcL, yvcJ |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
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Contents
Categories containing this gene/protein
carbon core metabolism, cell shape
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34760
Phenotypes of a mutant
- unable to grow with gluconeogenic substrates as single carbon source PubMed
- filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) PubMed, this is supressed by overexpression of MreB or by deletion of ponA (PBP1 ) PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: UPF0052 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: localized as a helical-like pattern PubMed
Database entries
- Structure:
- UniProt: O06974
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation: very weak stimuation of expression by citrate and succinate PubMed
- Regulatory mechanism:
- Additional information:
- translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
Biological materials
- Mutant:
- Expression vector: pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
- Antibody:
Labs working on this gene/protein
Boris Görke, University of Göttingen, Germany Homepage
Anne Galinier, University of Marseille, France
Your additional remarks
References
Reviews
Original publications
