Difference between revisions of "YvcK"

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(The gene)
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
** unable to grow with gluconeogenic substrates as single carbon source {{PubMed|16272399}}
+
* unable to grow with gluconeogenic substrates as single carbon source {{PubMed|16272399}}
** filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) {{PubMed|16272399}}, this is supressed by overexpression of [[MreB]] or by deletion of ''[[ponA]]'' ([[ponA|PBP1]] ) {{PubMed|21320184}}
+
* filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) {{PubMed|16272399}}, this is supressed by overexpression of [[MreB]] or by deletion of ''[[ponA]]'' ([[ponA|PBP1]] ) {{PubMed|21320184}}
 
=== Database entries ===
 
=== Database entries ===
  
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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=

Revision as of 19:47, 29 April 2013

  • Description: essential for growth under gluconeogenetic conditions, required for correct localization of PBP1

Gene name yvcK
Synonyms
Essential no
Product unknown
Function localization of PBP1
Gene expression levels in SubtiExpress: yvcK
MW, pI 34 kDa, 5.408
Gene length, protein length 951 bp, 317 aa
Immediate neighbours yvcL, yvcJ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YvcK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YvcK expression.png















Categories containing this gene/protein

carbon core metabolism, cell shape

This gene is a member of the following regulons

Efp-dependent proteins

The gene

Basic information

  • Locus tag: BSU34760

Phenotypes of a mutant

  • unable to grow with gluconeogenic substrates as single carbon source PubMed
  • filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) PubMed, this is supressed by overexpression of MreB or by deletion of ponA (PBP1 ) PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: UPF0052 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: very weak stimuation of expression by citrate and succinate PubMed
  • Regulatory mechanism:
  • Additional information:
    • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

  • Mutant:
  • Expression vector: pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
  • Antibody:

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Anne Galinier, University of Marseille, France

Your additional remarks

References

Reviews

PubMed

Original publications