Difference between revisions of "YvcK"

From SubtiWiki
Jump to: navigation, search
Line 49: Line 49:
  
  
 +
 +
= Categories containing this gene/protein =
 +
{{SubtiWiki category|[[carbon core metabolism]]}}
 
=The protein=
 
=The protein=
  

Revision as of 23:44, 30 November 2010

  • Description: essential for growth under gluconeogenetic conditions

Gene name yvcK
Synonyms
Essential no
Product unknown
Function unknown
MW, pI 34 kDa, 5.408
Gene length, protein length 951 bp, 317 aa
Immediate neighbours yvcL, yvcJ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YvcK context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU34760

Phenotypes of a mutant

    • unable to grow with gluconeogenic substrates as single carbon source PubMed
    • filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

Categories containing this gene/protein

carbon core metabolism

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: UPF0052 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: very weak stimuation of expression by citrate and succinate PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector: pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
  • Antibody:

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Anne Galinier, University of Marseille, France

Your additional remarks

References