yvaK

168

general stress protein, carboxylesterase

locus: BSU_33620

Molecular weight: 28.26 kDa

pI: 4.99

Protein length: 248 aa

Gene length: 747 bp

function: unknown

product: unknown

essential: no

ec: 3.1.1.1

synonyms: yvaK, est, fphH

List of homologs in different organisms, belongs to COG1647 (Galperin et al., 2021)

Gene

Coordinates: 3,454,221 → 3,454,967

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The protein

Catalyzed reaction/ biological activity

• carboxylic ester + H₂O --> carboxylate + alcohol + H⁺ (according to UniProt)

Protein family

• lipase/esterase LIP3/BchO family (single member, according to UniProt)

Structure

• [PDB|1TQH] (Geobacillus stearothermophilus)
• [PDB|8FTP] (Staphylococcus aureus) [pubmed|37824340]
• [AF|O32232]

Biological materials

Mutant

• MGNA-B606 (yvaK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1605 NBRP B. subtilis, Japan]
• BKE33620 (Δ[gene|C0B6520E74CE9F7EE3DEC8C0D83E0770F241C4E8|yvaK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTCATGTCTCCCTTTC,  downstream forward: _UP4_TGGTAATCAACAGGAGGTCG
• BKK33620 (Δ[gene|C0B6520E74CE9F7EE3DEC8C0D83E0770F241C4E8|yvaK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTCATGTCTCCCTTTC,  downstream forward: _UP4_TGGTAATCAACAGGAGGTCG

References

• 11544224,17369301,23812333,37824340