metN

168

methionine [wiki|ABC transporter ](ATP-binding protein)

locus: BSU_32750

Molecular weight: 37.73 kDa

pI: 7.3

Protein length: 341 aa

Gene length: 1026 bp

function: methionine uptake

product: methionine [wiki|ABC transporter ](ATP-binding protein)

essential: no

ec: 3.6.3.-

synonyms: metN, yusC

List of homologs in different organisms, belongs to COG1135 (Galperin et al., 2021)

Gene

Coordinates: 3,363,266 → 3,364,291

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The protein

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transporter domain] (aa 2-241) (according to UniProt)

Structure

• [PDB|3DHW] (the [protein|D7254B7F31D3F4CD18172AFB0F526CAE5195BDB6|metN]-[protein|F94070DD039A422055FFC35087FB87765F22E156|metP] complex from ''E. coli'', 44% identity) [Pubmed|18621668]
• [AF|O32169]

[wiki|Localization]

• associated to the cell membrane, via [protein|F94070DD039A422055FFC35087FB87765F22E156|metP] [Pubmed|10092453,18763711]

Expression and Regulation

Operons

• genes: [gene|D7254B7F31D3F4CD18172AFB0F526CAE5195BDB6|metN]-[gene|F94070DD039A422055FFC35087FB87765F22E156|metP]-[gene|0481A9C134A6EEC6F111AD0C03E2E345D6A315A0|metQ]

  description: [Pubmed|10094622]

  regulation

  • activated during growth in the presence of branched chain amino acids ([protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]) [Pubmed|12618455]
  • the mRNA is processed between [gene|F94070DD039A422055FFC35087FB87765F22E156|metP] and [gene|0481A9C134A6EEC6F111AD0C03E2E345D6A315A0|metQ] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
  • the [wiki|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

  regulatory mechanism

  • S-box: RNA switch, the [wiki|S-box] [wiki|riboswitch] binds S-adenosylmethionine resulting in termination, in [regulon|other_regulator:S-box|S-box]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: activation, [Pubmed|12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]

Biological materials

Mutant

• MGNA-A596 (yusC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/596 NBRP B. subtilis, Japan]
• 1A797 ( ''metN''::''cat''), [Pubmed|14990259], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A797&Search=1A797 BGSC]
• BKE32750 (Δ[gene|D7254B7F31D3F4CD18172AFB0F526CAE5195BDB6|metN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAAGCAATCACCTGCCTTA,  downstream forward: _UP4_CAGGTGAAAGCAGAGGTGAT
• BKK32750 (Δ[gene|D7254B7F31D3F4CD18172AFB0F526CAE5195BDB6|metN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAAGCAATCACCTGCCTTA,  downstream forward: _UP4_CAGGTGAAAGCAGAGGTGAT

References

• 36815589,10092453,14990259,12618455,18763711,10094622,18039762,18621668,29794222