besA

168

Ferri-bacillibactin esterase

locus: BSU_32010

Molecular weight: 35.34 kDa

pI: 8.2

Protein length: 289 aa

Gene length: 870 bp

function: iron acquisition

product: ferri-bacillibactin esterase

essential: no

synonyms: besA, yuiI

List of homologs in different organisms, belongs to COG2819 (Galperin et al., 2021)

Gene

Coordinates: 3,292,490  3,293,359

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The protein

Catalyzed reaction/ biological activity

• catalyses ferri-bacillibactin hydrolysis leading to cytosolic iron release [pubmed|28283524]

Protein family

• Esterase D family (single member, according to UniProt)

Structure

• [PDB|2QM0] (from B. cereus, 46% identity)
• [AF|O32102]

[wiki|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|71F490B2C3199734620724B51F8E3D7977C2D8D8|besA]

  description: [Pubmed|12354229]

  regulation

  • induced upon iron starvation ([protein|search|Fur]) [Pubmed|12354229]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]

  additional information

  • [protein|search|translation] is likely to require [protein|search|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]

Biological materials

Mutant

• MGNA-A650 (yuiI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/650 NBRP B. subtilis, Japan]
• BKE32010 ([gene|71F490B2C3199734620724B51F8E3D7977C2D8D8|besA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32010 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCAGTACGACATTCCTC,  downstream forward: _UP4_TGAGACATACTCAGCCTTGC
• BKK32010 ([gene|71F490B2C3199734620724B51F8E3D7977C2D8D8|besA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32010 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCAGTACGACATTCCTC,  downstream forward: _UP4_TGAGACATACTCAGCCTTGC

References

• 16889643,12354229,19673474,20817675,28283524