malK

malK
168

two-component sensor kinase, regulation of malate uptake

locus
BSU_31520
Molecular weight
58.76 kDa
pI
6.95
Protein length
Gene length
function
regulation of malate uptake
product
two-component sensor kinase
essential
no
ec
null
synonyms
malK, yufL

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3290 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,237,157  3,238,758
Phenotypes of a mutant
unable to use malate as the single carbon source [Pubmed|12949159]
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The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [protein|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
[wiki|Domains]
two transmembrane segments
[wiki|PAS domain] (aa 210-281) (according to UniProt)
[wiki|Histidine kinase domain] (aa 335-527) (according to UniProt)
Structure
[AF|O05250]
Modification
autophosphorylation on a His residue
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|6FBBC2BDEAD72BEA0D9EA56668FDA5A3BE6F694E|malK]-[gene|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR]
description
[Pubmed|22383849]
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[gene|6FBBC2BDEAD72BEA0D9EA56668FDA5A3BE6F694E|malK]→[gene|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR]

2024-03-27 20:09:12

Jstuelk

33

4d0af3dc685749cd25b72d25b2068820a4e80caa

A1CE44BB5BE01A01460705F1FB2C87CE7994208A

Biological materials
Mutant
MGNA-A634 (yufL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/634 NBRP B. subtilis, Japan]
BKE31520 ([gene|6FBBC2BDEAD72BEA0D9EA56668FDA5A3BE6F694E|malK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31520 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATAAAACTTCCTTACGT,  downstream forward: _UP4_CCGTACGAACCGAAGGAGGA
BKK31520 ([gene|6FBBC2BDEAD72BEA0D9EA56668FDA5A3BE6F694E|malK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31520 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATAAAACTTCCTTACGT,  downstream forward: _UP4_CCGTACGAACCGAAGGAGGA
labs
[wiki|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
References
10094672,12949160,12949159,12949159,12949160

6FBBC2BDEAD72BEA0D9EA56668FDA5A3BE6F694E

Page visits: 2778

Time of last update: 2024-03-28 01:22:39

Author of last update: Melvin.boenninger