pncB

168

nicotinamide phosphoribosyltransferase, nicotinate phosphoribosyltransferase

locus: BSU_31750

Molecular weight: 56.01 kDa

pI: 5.09

Protein length: 490 aa

Gene length: 1473 bp

function: NAD salvage and biosynthesis

product: nicotinamide phosphoribosyltransferase

essential: yes

ec: 2.4.2.11

synonyms: pncB, yueK

List of homologs in different organisms, belongs to COG1488 (Galperin et al., 2021)

Gene

Coordinates: 3,259,403  3,260,875

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• 5-phospho-α-D-ribose 1-diphosphate + ATP + H₂O + nicotinamide --> nicotinamide mononucleotide + ADP + diphosphate + phosphate [https://www.sciencedirect.com/science/article/pii/S1359511324000539#ab0020 paper]
• 5-phospho-α-D-ribose 1-diphosphate + ATP + H₂O + nicotinate --> ADP + diphosphate + nicotinate β-D-ribonucleotide + phosphate (according to UniProt)

Protein family

• NAPRTase family (single member, according to UniProt)

Structure

• [PDB|2F7F] (from ''Enterococcus faecalis'', 61% identity)
• [AF|O32090]

Expression and Regulation

Operons

• genes: [gene|26430DCCEC263E7D03A3BBA4E7D636A250C3208B|pncA]-[gene|7CA7ADDBDDE46B0E6522704FD19C0BCF6FA9E058|pncB]

  description: [Pubmed|22383849]

• genes: [gene|17F2619E74A1604E86ED94E1BEE683D49E437A6C|yueH]-[gene|3918D82756A64E86E96A21ACEB34B0CF992D662D|yueI]-[gene|26430DCCEC263E7D03A3BBA4E7D636A250C3208B|pncA]-[gene|7CA7ADDBDDE46B0E6522704FD19C0BCF6FA9E058|pncB]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B562 (yueK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1561 NBRP B. subtilis, Japan]

References

• 16199587,9274030