YtxJ

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  • Description: general stress protein

Gene name ytxJ
Synonyms csb40
Essential no
Product unknown
Function unknown
Gene expression levels in SubtiExpress: ytxJ
MW, pI 12 kDa, 5.089
Gene length, protein length 324 bp, 108 aa
Immediate neighbours aroA, ytxH
Sequences Protein DNA DNA_with_flanks
Genetic context
YtxJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YtxJ expression.png
























Categories containing this gene/protein

general stress proteins (controlled by SigB), phosphoproteins

This gene is a member of the following regulons

SigB regulon, SigH regulon

The gene

Basic information

  • Locus tag: BSU29760

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: Trx family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: contains a single conserved Cys residue in a motif (TCPIS) reminiscent of that found in monothiol glutaredoxins [3]
  • Modification: phosphorylation on (Thr-93 OR Ser-94 OR Ser-96 OR Thr-99) PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [4]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 933 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1969 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

YpdA, YqiW, YphP and YtxJ cooccur in those species predicted to synthesize bacillithiol.

References

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

D Varón, M S Brody, C W Price
Bacillus subtilis operon under the dual control of the general stress transcription factor sigma B and the sporulation transcription factor sigma H.
Mol Microbiol: 1996, 20(2);339-50
[PubMed:8733232] [WorldCat.org] [DOI] (P p)