refZ

168

regulator of [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] ([wiki|TetR family]), facilitates switch from medial to polar [protein|search|FtsZ ]ring placement during sporulation

locus: BSU_29630

Molecular weight: 24.24 kDa

pI: 6.71

Protein length: 207 aa

Gene length: 624 bp

function: relocalization of the [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] ring, placement of the sporulation septum

product: regulator of [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ]

essential: no

synonyms: refZ, yttP

List of homologs in different organisms, belongs to COG1309 (Galperin et al., 2021)

Gene

Coordinates: 3,032,417 → 3,033,040

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The protein

Catalyzed reaction/ biological activity

• facilitates switch from medial to polar [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] ring placement during [wiki|sporulation] [Pubmed|22730127]
• binds to specific DNA motifs (RBMs) that localize near the poles at the time of septation [pubmed|31160399]
• facilitates the timely assembly of polar Z-rings and partially defines the region of chromosome initially captured in the forespore [pubmed|31160399]
• a [gene|C5F67C3ACB06A5029B4AE69A065BC55622372404|refZ] [gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc] double mutant is defective in cell division and exhibits reduced sporulation [pubmed|35506695]

Protein family

• [wiki|TetR family]

[wiki|Domains]

• TetR-like DNA-binding helix-turn-helix domain at the N-terminus [Pubmed|22730127]
• [wiki|HTH tetR-type domain] (aa 3-63) (according to UniProt)

Structure

• [PDB|6MJ1]
• [AF|O34970]

[wiki|Localization]

• polar foci [Pubmed|22730127]

Expression and Regulation

Operons

• genes: [gene|C5F67C3ACB06A5029B4AE69A065BC55622372404|refZ]

  description: [Pubmed|22383849]

  regulation

  • expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|14762014]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|14762014], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]

Biological materials

Mutant

• MGNA-A426 (yttP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/426 NBRP B. subtilis, Japan]
• BKE29630 (Δ[gene|C5F67C3ACB06A5029B4AE69A065BC55622372404|refZ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACGGCTCACTCCTTCCT,  downstream forward: _UP4_AACTAGCACCGTTCCAAAAA
• BKK29630 (Δ[gene|C5F67C3ACB06A5029B4AE69A065BC55622372404|refZ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACGGCTCACTCCTTCCT,  downstream forward: _UP4_AACTAGCACCGTTCCAAAAA

References

Reviews

• 24006471

Original Publications

• 14651647,14762014,12850135,22730127,22383849,26360512,30092000,31160399,35506695