ytsJ

168

bifunctional malic/malolactic enzyme

locus: BSU_29220

Molecular weight: 43.51 kDa

pI: 5.05

Protein length: 410 aa

Gene length: 1233 bp

function: malate utilization, balancing of the NADPH pool

product: bifunctional malic/malolactic enzyme

essential: no

ec: 1.1.1.38

synonyms: ytsJ

List of homologs in different organisms, belongs to COG0281 (Galperin et al., 2021)

Gene

Coordinates: 2,989,900 2,991,132

Phenotypes of a mutant

• Poor growth with malate as single carbon source [Pubmed|16788182]

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The protein

Catalyzed reaction/ biological activity

• (S)-malate + NADP⁺ --> CO₂ + NADPH + pyruvate [pubmed|33824210]
• malate --> lactate + CO₂ [pubmed|33824210]

Protein family

• [wiki|malic enzymes family] (according to UniProt)

[wiki|Cofactors]

• NADP⁺

Structure

• [PDB|1WW8] (from ''Pyrococcus horikoshii'', 46% identity, 63% similarity)
• [AF|O34962]

Modification

• phosphorylated on Arg-27 [Pubmed|31221751]

Effectors of protein activity

• in the presence of excess NADPH, the enzyme switches from oxidative pyruvate formation to non-oxidative lactate formation [pubmed|33824210]

Paralogous protein(s)

• [protein|BBBCD5F56779895189E21076AF165B901F654534|malS], [protein|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]

[wiki|Localization]

• Cytoplasm (Homogeneous) [Pubmed|16479537]

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• MGNA-A176 (ytsJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/176 NBRP B. subtilis, Japan]
• GP612 (spc), available in [wiki|Jrg Stlke]'s lab
• GP1143 (spc), available in [wiki|Jrg Stlke]'s lab
• BKE29220 ([gene|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|ytsJ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCTAAACACTCCTT, downstream forward: _UP4_TAATTTTAATTCATTCCAAA
• BKK29220 ([gene|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|ytsJ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCTAAACACTCCTT, downstream forward: _UP4_TAATTTTAATTCATTCCAAA

FLAG-tag construct

• GP1131 (spc, based on [wiki|pGP1331]), available in [wiki|Jrg Stlke]'s lab [pubmed|20933603]

GFP fusion

• GP1432 (spc, based on [wiki|pGP1870]), available in [wiki|Jrg Stlke]'s lab

labs

• [wiki|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

• 16479537,16788182,12949160,9387221,23136871,22740702,15378759,31221751,33824210