thiI

thiI
168

sulfuryl transferase, biosynthesis of 4-thiouridine in tRNA

locus
BSU_29580
Molecular weight
40.17 kDa
pI
9.66
Protein length
Gene length
function
biosynthesis of 4-thiouridine in tRNA
product
sulfuryl transferase
essential
no
synonyms
thiI, ytbJ

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0301 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,025,748  3,026,953
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The protein
Catalyzed reaction/ biological activity
receives a sulfuryl group from [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ] and transfers it to uridine  at position 8 of tRNA  [Pubmed|22773787]
[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine +  uridine in tRNA + ATP + H+ + 2 reduced [2Fe-2S]-[ferredoxin] --> [ThiI sulfur-carrier protein]-L-cysteine +  4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)
[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] --> [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H+ + L-cysteinyl-[cysteine desulfurase] (according to UniProt)
Protein family
thiI family (single member, according to UniProt)
[wiki|Domains]
THUMP domain (aa 60-165) (according to UniProt)
Structure
[PDB|4KR6] (ThiI-RNA complex from Thermotoga maritima) [pubmed|24705700]
[PDB|2C5S] (from B. anthracis) [pubmed|16343540]
[AF|O34595]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]-[gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]-[gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]
description
[Pubmed|22383849]
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[gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]→[gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]

2024-03-16 12:19:00

Jstuelk

76

c96a207b604fba3a97ff9f805c130b9d09817187

28FE39136DED7BC0B197A71666940376494D0F7A

Biological materials
Mutant
BKE29580 ([gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TAATATATGATCGTAATTCA,  downstream forward: _UP4_TAAATCAATTTTCAGCTCCT
BKK29580 ([gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TAATATATGATCGTAATTCA,  downstream forward: _UP4_TAAATCAATTTTCAGCTCCT
References
14567704,22773787,16343540,24705700

A64A59C2B15015F13911EC53763D370F780400FD

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Time of last update: 2024-03-28 15:29:23

Author of last update: Melvin.boenninger