YtaG

• Description: dephospho-CoA kinase
  
  

Gene name	ytaG
Synonyms	coaE
Essential	yes PubMed
Product	dephospho-CoA kinase
Function	synthesis of coenzyme A
Gene expression levels in SubtiExpress: ytaG
Metabolic function and regulation of this protein in SubtiPathways: ytaG
MW, pI	21 kDa, 4.858
Gene length, protein length	591 bp, 197 aa
Immediate neighbours	ytbE, ytaF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, essential genes, phosphoproteins, sporulation/ other

The gene

Basic information

• Locus tag: BSU29060

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU29060

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + 3'-dephospho-CoA = ADP + CoA (according to Swiss-Prot)

• Protein family: coaE family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU29060

• Structure: 1N3B (from Escherichia coli, 40% identity, 60% similarity) PubMed

• UniProt: O34932

• KEGG entry: [3]

• E.C. number: 2.7.1.24

Expression and regulation

• Operon: ytaG PubMed

• Expression browser: ytaG PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 144 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 307 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alain Lévine, Françoise Vannier, Cédric Absalon, Lauriane Kuhn, Peter Jackson, Elaine Scrivener, Valérie Labas, Joëlle Vinh, Patrick Courtney, Jérôme Garin, Simone J Séror
Analysis of the dynamic Bacillus subtilis Ser/Thr/Tyr phosphoproteome implicated in a wide variety of cellular processes.
Proteomics: 2006, 6(7);2157-73
[PubMed:16493705] [WorldCat.org] [DOI] (P p)

Nicholas O'Toole, João A R G Barbosa, Yunge Li, Li-Wei Hung, Allan Matte, Miroslaw Cygler
Crystal structure of a trimeric form of dephosphocoenzyme A kinase from Escherichia coli.
Protein Sci: 2003, 12(2);327-36
[PubMed:12538896] [WorldCat.org] [DOI] (P p)

P Mishra, P K Park, D G Drueckhammer
Identification of yacE (coaE) as the structural gene for dephosphocoenzyme A kinase in Escherichia coli K-12.
J Bacteriol: 2001, 183(9);2774-8
[PubMed:11292795] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)