ytaF

168

[wiki|sporulation] protein

locus: BSU_29070

Molecular weight: 17.23 kDa

pI: 9.79

Protein length: 210 aa

Gene length: 633 bp

function: unknown

product: [wiki|sporulation] protein

essential: no

synonyms: ytaF

List of homologs in different organisms, belongs to COG1971 (Galperin et al., 2021)

Gene

Coordinates: 2,971,531  2,972,163

Phenotypes of a mutant

• strongly reduced [wiki|sporulation] efficiency (10% as compared to wild type) [Pubmed|23396918]
• block in stage III of [wiki|sporulation] (engulfment of the forespore) [Pubmed|23396918]

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The protein

[wiki|Domains]

• 4 potential transmembrane domains [Pubmed|23396918]

Structure

• [AF|C0SP79]

[wiki|Localization]

• cell membrane (according to UniProt)

Biological materials

Mutant

• MGNA-A117 (ytaF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/117 NBRP B. subtilis, Japan]
• BKE29070 ([gene|3BEE8E248BC583FCCB37B7A209A122DD93CD398C|ytaF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29070 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCCGTTTCTTCCTTTC,  downstream forward: _UP4_TAAAAAGGAGTGGATCTCTT
• BKK29070 ([gene|3BEE8E248BC583FCCB37B7A209A122DD93CD398C|ytaF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29070 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCCGTTTCTTCCTTTC,  downstream forward: _UP4_TAAAAAGGAGTGGATCTCTT

References

• 23396918,35638784