thrR

thrR
168

transcription repressor of threonine biosynthetic genes

locus
BSU_27910
Molecular weight
16.52 kDa
pI
6.36
Protein length
Gene length
function
control of threonine biosynthesis
product
transcription repressor  of threonine biosynthetic genes
essential
no
synonyms
thrR, pheB, yszB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4492 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,852,157  2,852,600
Phenotypes of a mutant
resistance to serine [pubmed|32743959]
Visit Sartorius.com Visit Sartorius.com
Advertisement
The protein
Catalyzed reaction/ biological activity
control of the threonine biosynthetic genes (''[gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]-[gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]-[gene|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]'' and ''[gene|548E92526BC00999031D6EDE43FE061CE0448AFC|thrD]'') [Pubmed|27260660]
Protein family
UPF0735 family (single member, according to UniProt)
[wiki|Domains]
putative DNA binding signature (aa 19-51)
[wiki|ACT domain] (aa 70-145) (according to UniProt)
Structure
[AF|P21204]
Effectors of protein activity
lysine and cysteine (indirectly) inhibit DNA-binding activity of ThrR  [Pubmed|27260660]
Expression and Regulation
Operons
genes
[gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]-[gene|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg]-[gene|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]-[gene|D4CCF6727AAC048792E59717E588C36EB617249D|pheA]
description
[Pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2537815], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
expression is repressed by binding of [wiki|RpoE] to the A-rich sequence in the -35 region of the promoter [Pubmed|27679485]
Open in new tab

[gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]→[gene|D4CCF6727AAC048792E59717E588C36EB617249D|pheA]

2024-02-06 17:10:53

ghost

62

22e8673d70be078a28f38f2ef0353054aac7a361

EFF1EF2402AB3F0071535ACBC9F9FB0A68987C02

Biological materials
Mutant
BKE27910 (''[gene|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]''::''erm'', available in the BGSC and in [wiki|Jörg Stülke]'s and [wiki|Fabian Commichau]'s labs) [Pubmed|27260660]
BKE27910 ([gene|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGCATCCCCCCTTTAA,  downstream forward: _UP4_GGTGCATAAGGGAGAGAAAA
BKK27910 ([gene|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGCATCCCCCCTTTAA,  downstream forward: _UP4_GGTGCATAAGGGAGAGAAAA
Expression vectors
pGP2290: expression of ''thrR'' in B. subtilis (in[wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
pBP323 (N-terminal Strep-tag, in [wiki|pGP172]) (available in [wiki|Fabian Commichau]'s lab) [Pubmed|27260660]
pBP620 (N-terminal Strep-tag, for [wiki|SPINE], purification from ''B. subtilis'', in [wiki|pGP380]) (available in [wiki|Fabian Commichau]'s lab) [Pubmed|27260660]
pBP622 (C-terminal Strep-tag, for [wiki|SPINE], purification from ''B. subtilis'', in [wiki|pGP382]) (available in [wiki|Fabian Commichau]'s lab) [Pubmed|27260660]
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Fabian Commichau]'s lab [Pubmed|27260660]
lacZ fusion
pGP2291 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Fabian Commichau] Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]
References
2537815,25777134,27260660,32743959

A4B7CF7A1C704C750AFAD97A43AF975260935083

Page visits: 4762

Time of last update: 2024-03-28 22:11:45

Author of last update: Jstuelk