yrrO

yrrO
168

tRNA uridine hydroxylase (in complex with [protein|A3EC327DA14F188E88AF4221B51EE5950F882277|yrrN])

locus
BSU_27340
Molecular weight
47.46 kDa
pI
4.85
Protein length
Gene length
function
introduction of 5-methoxyuridine modification in tRNA (U34)
product
tRNA uridine hydroxylase
essential
no
ec
null
synonyms
yrrO, trhP2

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0826 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,792,860 → 2,794,128
Phenotypes of a mutant
50% reduction in mo5U tRNA levels [pubmed|31358606]
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The protein
Catalyzed reaction/ biological activity
hydroxylation of U34 in tRNA to give 5-hydroxyuridine (ho5U) (in complex with [protein|A3EC327DA14F188E88AF4221B51EE5950F882277|yrrN]) with prephenate as hydroxyl donor, ho5U is the precursor for 5-methoxyuridine modification (by [protein|CE4F2965077F2A5882432D683520E09B70B9369A|trmR])  [pubmed|31358606]
Protein family
peptidase U32 family (with [protein|A3EC327DA14F188E88AF4221B51EE5950F882277|yrrN], according to UniProt)
Structure
[AF|O32034]
Paralogous protein(s)
[protein|A3EC327DA14F188E88AF4221B51EE5950F882277|yrrN]
Expression and Regulation
Operons
genes
[gene|CE4F2965077F2A5882432D683520E09B70B9369A|trmR]-[gene|A3EC327DA14F188E88AF4221B51EE5950F882277|yrrN]-[gene|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|yrrO]-[gene|A659D9894E777D8A99707CB750DF2918AEE06CE9|udk]-[gene|6FBF27C12B4B3DC2CE373E5199357042114471F8|greA]
description
[Pubmed|22383849]
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[gene|CE4F2965077F2A5882432D683520E09B70B9369A|trmR]→[gene|6FBF27C12B4B3DC2CE373E5199357042114471F8|greA]

2024-03-21 13:39:38

Jstuelk

142

0a66c040f2f5a1d47c0559c4047af2666d30c835

5807E821D8403F7EC565573C8910C732811F8BB4

Biological materials
Mutant
MGNA-A007 (yrrO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/7 NBRP B. subtilis, Japan]
BKE27340 (Δ[gene|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|yrrO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27340 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGCTTAACCTCCTTTTG,  downstream forward: _UP4_ATGAGAAAGGGGAAGTAACA
BKK27340 (Δ[gene|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|yrrO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27340 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGCTTAACCTCCTTTTG,  downstream forward: _UP4_ATGAGAAAGGGGAAGTAACA
References
Research papers
36815589,31358606

D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D

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Time of last update: 2024-03-27 23:11:42

Author of last update: Jstuelk