trmR

168

tRNA wobble uridine modifying methyltransferase

locus: BSU_27360

Molecular weight: 24.92 kDa

pI: 5.57

Protein length: 217 aa

Gene length: 654 bp

function: introduction of 5-methoxyuridine modification in tRNA (U34)

product: tRNA wobble uridine modifying methyltransferase

essential: no

synonyms: trmR, yrrM

List of homologs in different organisms, belongs to COG4122 (Galperin et al., 2021)

Gene

Coordinates: 2,795,082 → 2,795,735

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The protein

Catalyzed reaction/ biological activity

• methylation of (5-hydroxyuridine) ho⁵U containing tRNA to 5-methoxyuridine (mo⁵U) [pubmed|29982645]
• 5-hydroxyuridine³⁴ in tRNA + S-adenosyl-L-methionine --> 5-methoxyuridine³⁴ in tRNA + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• [wiki|Methyltransferase superfamily] (according to UniProt)
• [wiki|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)

[wiki|Cofactors]

• SAM [pubmed|29982645]

Structure

• [PDB|5ZW3] [pubmed|29982645]
• [PDB|5ZW4] (bound to tRNA) [pubmed|29982645]
• [AF|O32036]

Biological materials

Mutant

• MGNA-A853 (yrrM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/853 NBRP B. subtilis, Japan]
• BKE27360 (Δ[gene|CE4F2965077F2A5882432D683520E09B70B9369A|trmR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTACAAACAGCCTCCCGT,  downstream forward: _UP4_AGTAAAAAGAAGAGGTGAAC
• BKK27360 (Δ[gene|CE4F2965077F2A5882432D683520E09B70B9369A|trmR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTACAAACAGCCTCCCGT,  downstream forward: _UP4_AGTAAAAAGAAGAGGTGAAC

References

Research papers

• 29982645,31358606