mccB

168

cystathionine lyase/ homocysteine gamma-lyase

locus: BSU_27250

Molecular weight: 40.73 kDa

pI: 5.22

Protein length: 379 aa

Gene length: 1137 bp

function: methionine-to-cysteine conversion

product: cystathionine lyase/  homocysteine gamma-lyase

essential: no

ec: 4.4.1.1

synonyms: mccB, yrhB

List of homologs in different organisms, belongs to COG0626 (Galperin et al., 2021)

Gene

Coordinates: 2,785,001 → 2,786,140

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The protein

Catalyzed reaction/ biological activity

• H₂O + L,L-cystathionine --> 2-oxobutanoate + L-cysteine + NH₄⁺ (according to UniProt)
• H₂O + L-homocysteine --> 2-oxobutanoate + H⁺ + hydrogen sulfide + NH₄⁺ (according to UniProt)

Protein family

• trans-sulfuration enzymes family (with [protein|04DC792180FDC5E7916F2DBB2EC2C34369202FE0|metI] and [protein|1C0D38F23D3A2CAA1EB057A92D6F571D0D2AA724|metC], according to UniProt)

[wiki|Cofactors]

• PLP (according to UniProt)

Structure

• [PDB|4L0O] (from Helicobacter pylori, 61% identity)
• [AF|O05394]

Paralogous protein(s)

• [protein|04DC792180FDC5E7916F2DBB2EC2C34369202FE0|metI], [protein|1C0D38F23D3A2CAA1EB057A92D6F571D0D2AA724|metC]

[wiki|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|992A24CF9492965BF0E9EEFBB6F0A06871275068|yrrT]-[gene|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|mtnN]-[gene|F195C47849F4F6B4C56E3A386F5D8C84326B01C4|mccA]-[gene|314CB4923D15140E72B0ABAFE3E8BE9CA7310E10|mccB]-[gene|177EFFDCA6E3C3BB840EED13D9F2D71F99B833B6|yrhC]

  description: [Pubmed|17056751]

  regulation

  • repressed in the presence of cysteine ([protein|search|CymR]) [Pubmed|16513748]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16513748], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|12642660,16885442], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16513748], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-A848 (yrhB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/848 NBRP B. subtilis, Japan]
• 1A942 ( ''mccB''::''kan''), [Pubmed|17056751], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A942&Search=1A942 BGSC]
• 1A945 ( ''mccB''::''kan''), [Pubmed|17056751], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A945&Search=1A945 BGSC]
• BKE27250 (Δ[gene|314CB4923D15140E72B0ABAFE3E8BE9CA7310E10|mccB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TATCATCAATGTTTTTTTCT,  downstream forward: _UP4_TAACAAATGGATAGAATTAC
• BKK27250 (Δ[gene|314CB4923D15140E72B0ABAFE3E8BE9CA7310E10|mccB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TATCATCAATGTTTTTTTCT,  downstream forward: _UP4_TAACAAATGGATAGAATTAC

References

• 17056751,16513748,16885442,12642660