Difference between revisions of "YraA"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''[[adhA]]-[[yraA]]'' {{PubMed|19170879}}
  
 
* '''[[Sigma factor]]:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed],  [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed]
 
* '''[[Sigma factor]]:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed],  [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed]
  
* '''Regulation:'''  induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]
+
* '''Regulation:'''   
 +
** induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]
 +
** induced in the presence of the toxic carbonyls methylglyoxal and formaldehyde ([[AdhR]]) {{PubMed|19170879}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[AdhR]]: transcription activation {{PubMed|19170879}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 20:30, 25 November 2009

  • Description: general stress protein, degradation of damaged thiol-containing proteins

Gene name yraA
Synonyms
Essential no
Product cysteine proteinase
Function degradation of damaged thiol-containing proteins
MW, pI 18 kDa, 4.729
Gene length, protein length 507 bp, 169 aa
Immediate neighbours adhA, sacC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YraA context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Locus tag: BSU27020

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: peptidase C56 family (according to Swiss-Prot)
  • Paralogous protein(s): YfkM

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by stress (SigB) PubMed
    • induced in the presence of the toxic carbonyls methylglyoxal and formaldehyde (AdhR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Thi Thu Huyen Nguyen, Warawan Eiamphungporn, Ulrike Mäder, Manuel Liebeke, Michael Lalk, Michael Hecker, John D Helmann, Haike Antelmann
Genome-wide responses to carbonyl electrophiles in Bacillus subtilis: control of the thiol-dependent formaldehyde dehydrogenase AdhA and cysteine proteinase YraA by the MerR-family regulator YraB (AdhR).
Mol Microbiol: 2009, 71(4);876-94
[PubMed:19170879] [WorldCat.org] [DOI] (I p)

Adrian J Jervis, Penny D Thackray, Chris W Houston, Malcolm J Horsburgh, Anne Moir
SigM-responsive genes of Bacillus subtilis and their promoters.
J Bacteriol: 2007, 189(12);4534-8
[PubMed:17434969] [WorldCat.org] [DOI] (P p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)