polY2

168

translesion synthesis (TLS-) DNA polymerase Y2

locus: BSU_23710

Molecular weight: 45.78 kDa

pI: 9.53

Protein length: 412 aa

Gene length: 1239 bp

function: UV-targeted mutagenesis

product: translesion synthesis (TLS-) DNA polymerase Y2

essential: no

synonyms: polY2, yqjW

List of homologs in different organisms, belongs to COG0389 (Galperin et al., 2021)

Gene

Coordinates: 2,464,562 → 2,465,800

Phenotypes of a mutant

• sensitive to blue light-induced DNA damage [pubmed|30054368]
• increased sensitivity to Cr(VI) [pubmed|30745368]

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The protein

Catalyzed reaction/ biological activity

• 2'-deoxyribonucleoside 5'-triphosphate + DNA_(n) --> diphosphate + DNA_(n+1) (according to UniProt)
• required for Cr(VI)-induced mutagenesis [pubmed|30745368]

Protein family

• [wiki|DNA polymerase type-Y family] (according to UniProt)

[wiki|Domains]

• [wiki|UmuC domain] (aa 7-192) (according to UniProt)

Structure

• [PDB|4DEZ] (from Mycobacterium smegmatis, 30% identity) [pubmed|22868761]
• [AF|P54560]

Paralogous protein(s)

• [protein|C87D33852F263A553CA747608E6200E4AD8344B5|polY1], [protein|BE8D5E038C0FD748C0F9C3F18DCA601BFFE8EA52|uvrX]

Expression and Regulation

Operons

• genes: [gene|F0BC150F59730E64BA9AB3F979544EF6B5CC4E9E|polY2]-[gene|9BC3DF46C944A0FD5CAAB4513C4AF00EFC5FC8D5|yqjX]-[gene|5B8A6D282E34683726BD6C36E09A6C36E8F99795|yqjY]-[gene|9F88AC3A792F4EE8AA853849BBCF5065717B1D8B|yqjZ]-[gene|3ECD25A4ED9556E74112285B11FC180A7091D730|yqkA]-[gene|DC1C1D4BE1A7F4E28792A2B8273AFE2C7F90E58C|yqkB]-[gene|30363923724EBD39C27967B25B486C71453190D1|yqkC]

  description: [Pubmed|22383849]

  regulation

  • induced upon DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]) [Pubmed|15469515,16267290]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|15469515,16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]

Biological materials

Mutant

• MGNA-C403 (yqjW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2401 NBRP B. subtilis, Japan]
• GP1505 (''polY2''::''kan''), available in [wiki|Stülke] lab [pubmed|31948638]
• BKE23710 (Δ[gene|F0BC150F59730E64BA9AB3F979544EF6B5CC4E9E|polY2]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACACACCTCTAAAAAGA,  downstream forward: _UP4_GCTAAAATAGGGGGGCATTA
• BKK23710 (Δ[gene|F0BC150F59730E64BA9AB3F979544EF6B5CC4E9E|polY2]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACACACCTCTAAAAAGA,  downstream forward: _UP4_GCTAAAATAGGGGGGCATTA

References

Reviews

• 22933559,35210609,38052445,38551349

Original Publications

• 16267290,12644484,15469515,16045613,19924481,23686288,30054368,30096406,22868761,30745368,30916324,31948638