polY1

168

translesion synthesis (TLS-) DNA polymerase Y1, important for stationary phase mutagenesis, promotes efficient replisome progression through lagging-strand genes, thereby reducing potentially detrimental breaks and single-stranded DNA at these loci

locus: BSU_23870

Molecular weight: 46.86 kDa

pI: 9.95

Protein length: 414 aa

Gene length: 1245 bp

function: generation of mutations in stationary phase

product: translesion synthesis (TLS-) DNA polymerase Y1

essential: no

ec: 2.7.7.7

synonyms: polY1, yqjH

List of homologs in different organisms, belongs to COG0389 (Galperin et al., 2021)

Gene

Coordinates: 2,482,269 → 2,483,513

Phenotypes of a mutant

• reduced UV-induced mutagenesis [Pubmed|12644484]
• sensitive to blue light-induced DNA damage [pubmed|30054368]
• increased sensitivity to Cr(VI) [pubmed|30745368]

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The protein

Catalyzed reaction/ biological activity

• 2'-deoxyribonucleoside 5'-triphosphate + DNA_(n) --> diphosphate + DNA_(n+1) (according to UniProt)
• required for Cr(VI)-induced mutagenesis [pubmed|30745368]

Protein family

• [wiki|DNA polymerase type-Y family] (according to UniProt)

[wiki|Domains]

• [wiki|UmuC domain] (aa 8-189) (according to UniProt)

Structure

• [PDB|4IRC] (from E. coli, 35% identity) [pubmed|23525461]
• [AF|P54545]

Paralogous protein(s)

• [protein|F0BC150F59730E64BA9AB3F979544EF6B5CC4E9E|polY2], [protein|BE8D5E038C0FD748C0F9C3F18DCA601BFFE8EA52|uvrX]

[wiki|Localization]

• cytoplasm (homogeneous)

Expression and Regulation

Operons

• genes: [gene|C87D33852F263A553CA747608E6200E4AD8344B5|polY1]-[gene|61B7C51EB7E74226890010B61D8E41C02189A453|gndA]

  description: [pubmed|22383849]

  regulation

  • constitutively expressed [Pubmed|15469515]

Biological materials

Mutant

• MGNA-C390 (yqjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2388 NBRP B. subtilis, Japan]
• GP1111 (spc), available in [wiki|Jörg Stülke]'s lab [pubmed|31948638]
• BKE23870 (Δ[gene|C87D33852F263A553CA747608E6200E4AD8344B5|polY1]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23870 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAACAAATCTCCTTTTC,  downstream forward: _UP4_TGAATCGCTTGAAAAAAAGG
• BKK23870 (Δ[gene|C87D33852F263A553CA747608E6200E4AD8344B5|polY1]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23870 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAACAAATCTCCTTTTC,  downstream forward: _UP4_TGAATCGCTTGAAAAAAAGG

References

Reviews

• 22933559,29856930,35210609,38052445

Original Publications

• 36815589,12644484,15469515,16045613,19924481,23686288,25713353,30054368,30096406,23525461,30745368,30916324,31948638