YqiQ

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  • Description: methylisocitrate lyase

Gene name yqiQ
Synonyms prpB
Essential no
Product methylisocitrate lyase
Function mother cell metabolism
Metabolic function and regulation of this protein in SubtiPathways:
Coenzyme A, Central C-metabolism
MW, pI 32 kDa, 5.279
Gene length, protein length 903 bp, 301 aa
Immediate neighbours yqzF, mmgE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YqiQ context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

sporulation proteins, membrane proteins, poorly characterized/ putative enzymes

This gene is a member of the following regulons

CcpA regulon, SigE regulon

The gene

Basic information

  • Locus tag: BSU24120

Expression

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate (according to Swiss-Prot)
  • Protein family: Methylisocitrate lyase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1MUM (enzyme of E. coli), 3KZ2 (the enzyme from B. anthracis)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed early during sporulation in the mother cell (SigE) PubMed
    • repressed by glucose (CcpA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Clemens Grimm, Andreas Evers, Matthias Brock, Claudia Maerker, Gerhard Klebe, Wolfgang Buckel, Klaus Reuter
Crystal structure of 2-methylisocitrate lyase (PrpB) from Escherichia coli and modelling of its ligand bound active centre.
J Mol Biol: 2003, 328(3);609-21
[PubMed:12706720] [WorldCat.org] [DOI] (P p)

E M Bryan, B W Beall, C P Moran
A sigma E dependent operon subject to catabolite repression during sporulation in Bacillus subtilis.
J Bacteriol: 1996, 178(16);4778-86
[PubMed:8759838] [WorldCat.org] [DOI] (P p)