YqiQ

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  • Description: methylisocitrate lyase

Gene name yqiQ
Synonyms prpB
Essential no
Product methylisocitrate lyase
Function mother cell metabolism
Metabolic function and regulation of this protein in SubtiPathways:
Coenzyme A, Central C-metabolism
MW, pI 32 kDa, 5.279
Gene length, protein length 903 bp, 301 aa
Immediate neighbours yqzF, mmgE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YqiQ context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU24120

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate (according to Swiss-Prot)
  • Protein family: Methylisocitrate lyase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure: 1MUM (enzyme of E. coli)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation: repressed by glucose (CcpA)
  • Regulatory mechanism: CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Clemens Grimm, Andreas Evers, Matthias Brock, Claudia Maerker, Gerhard Klebe, Wolfgang Buckel, Klaus Reuter
Crystal structure of 2-methylisocitrate lyase (PrpB) from Escherichia coli and modelling of its ligand bound active centre.
J Mol Biol: 2003, 328(3);609-21
[PubMed:12706720] [WorldCat.org] [DOI] (P p)